Codeletion of p15 and p16 genes in primary non-small cell lung carcinoma

Chromosome band 9p21 is deleted frequently in non-small cell lung carcinoma (NSCLC), and the p15 and p16 cyclin-dependent kinase-4 inhibitor genes map within this deletion region. Recent studies demonstrated deletion of p15 and p16 in NSCLC metastases and cell lines, suggesting a role for these gene...

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Bibliographic Details
Published in:Cancer research (Chicago, Ill.) Ill.), 1995-07, Vol.55 (14), p.2968-2971
Main Authors: SHENG XIAO, DAIZONG LI, CORSON, J. M, VIJG, J, FLETCHER, J. A
Format: Article
Language:English
Subjects:
Adult
Aged
Alleles
Base Sequence
Biological and medical sciences
Carcinoma, Non-Small-Cell Lung - genetics
Carrier Proteins - genetics
Cell Cycle Proteins
Cyclin-Dependent Kinase Inhibitor p15
Cyclin-Dependent Kinase Inhibitor p16
Female
Gene Deletion
Gene Expression Regulation, Neoplastic
Humans
In Situ Hybridization, Fluorescence
Lung Neoplasms - genetics
Male
Medical sciences
Middle Aged
Molecular Sequence Data
Pneumology
Point Mutation
Sequence Analysis, DNA
Transcription Factors - genetics
Tumor Suppressor Proteins
Tumors of the respiratory system and mediastinum
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Summary:Chromosome band 9p21 is deleted frequently in non-small cell lung carcinoma (NSCLC), and the p15 and p16 cyclin-dependent kinase-4 inhibitor genes map within this deletion region. Recent studies demonstrated deletion of p15 and p16 in NSCLC metastases and cell lines, suggesting a role for these genes in NSCLC progression. We now report p15 and p16 copy number, as determined by fluorescence in situ hybridization with a P1 contig, in 18 primary NSCLCs. Codeletion of p15 and p16 was found in 15 of 18 NSCLCs, and 1 of the 3 tumors with normal p15 and p16 copy number had a nonsense mutation in exon 2 of p16. We conclude that p15 and p16 are deleted and/or mutated in most primary NSCLCs. Two observations, however, support the involvement of at least one additional tumor suppressor gene on chromosome 9. These observations are: (a) the large size (> 100 kb) of most NSCLC p15/p16 deletions; and (b) the absence of exon 2 mutations in most retained NSCLC p15 and p16 alleles.
ISSN:0008-5472
1538-7445