The mitochondrial genotype can influence nuclear gene expression in yeast

Isochromosomal, respiratory-deficient yeast strains, such as a mit$^{-}$, a hypersuppressive petite, and a petite lacking mitochondrial DNA, are phenotypically identical in spite of differences in their mitochondrial genomes. Subtractive hybridizations of complementary DNA's to polyadenylated R...

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Bibliographic Details
Published in:Science (American Association for the Advancement of Science) 1987-01, Vol.235 (4788), p.576-580
Main Authors: Parikh, V.S, Morgan, M.M, Scott, R, Clements, L.S, Butow, R.A
Format: Article
Language:English
Subjects:
Actins
ADN
ARN
Base Sequence
Biological and medical sciences
Cell Nucleus - physiology
Cell regulation
Cellular control mechanisms
Complementary DNA
Cytochrome c Group - genetics
Cytochromes
DNA
DNA probes
DNA, Fungal - genetics
DNA, Mitochondrial - genetics
Electron Transport Complex IV - genetics
Fundamental and applied biological sciences. Psychology
GENE
Gene expression
Gene Expression Regulation
GENES
Genes, Fungal
Genetics
Genomes
GENOTIPOS
GENOTYPE
GENOTYPES
MITOCHONDRIA
Mitochondria - physiology
Mitochondrial DNA
MITOCHONDRIE
MITOCONDRIA
Molecular and cellular biology
Molecular genetics
Mutation
Plasmids
Protein biosynthesis
Protein synthesis
RNA
RNA, Fungal - genetics
RNA, Messenger - genetics
RNA, Ribosomal - genetics
SACCHAROMYCES CEREVISIAE
Saccharomyces cerevisiae - genetics
Yeasts
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Summary:Isochromosomal, respiratory-deficient yeast strains, such as a mit$^{-}$, a hypersuppressive petite, and a petite lacking mitochondrial DNA, are phenotypically identical in spite of differences in their mitochondrial genomes. Subtractive hybridizations of complementary DNA's to polyadenylated RNA isolated from derepressed cultures of these strains reveal the presence of nuclear-encoded transcripts whose abundance varies not only between them and their respiratory-competent parent, but among the respiratory-deficient strains themselves. Transcripts of some nuclear-encoded mitochondrial proteins, like cytochrome c and the $\alpha $ and $\beta $ subunits of the mitochondrial adenosine triphosphatase, whose abundance is affected by glucose or heme, do not vary. In the absence of major metabolic variables, yeast cells seem to respond to the quality and quantity of mitochondrial DNA and modulate the levels of nuclear-encoded RNA's, perhaps as a means of intergenomic regulation.
ISSN:0036-8075
1095-9203
DOI:10.1126/science.3027892