Genetic analyses of tattered, an X-linked dominant, developmental mouse mutation

Tattered (Td) is an X-linked dominant mouse mutation that causes prenatal lethality in affected males. To map the locus, we analyzed 199 normal male and affected female progeny from a backcross of Td and Mus castaneus. Pedigree analysis of these animals suggests a gene order of cen-DXWas70-(Td, DXMi...

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Bibliographic Details
Published in:Mammalian genome 1995-04, Vol.6 (4), p.291-294
Main Authors: Merrell, K, Gonzales, J C, Wells, S, Calame, K, Herman, G E
Format: Article
Language:English
Subjects:
Animals
Blotting, Western
Chromosome Mapping
Crosses, Genetic
Female
Flow Cytometry
Genes, Lethal
Genetic Linkage
Haplotypes
Heterozygote
Male
Mice
Mice, Inbred Strains
Muridae - genetics
Mutation
Polymerase Chain Reaction
X Chromosome
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Summary:Tattered (Td) is an X-linked dominant mouse mutation that causes prenatal lethality in affected males. To map the locus, we analyzed 199 normal male and affected female progeny from a backcross of Td and Mus castaneus. Pedigree analysis of these animals suggests a gene order of cen-DXWas70-(Td, DXMit26, Gata1, Tcfe3)-(Cybb, Otc)-tel, where Tcfe3 is a transcription factor homologous to a gene involved in the murine microphthalmia (mi) mutation [Hodgkinson et al. Cell 74, 395-404, 1993]. To evaluate Tcfe3 as a candidate for Td, heterozygous tattered females were crossed to xid males to obtain females in which > 95% of B cells expressed genes solely from the Td X Chromosome (Chr). Fluorescent activated cell sorting (FACS) analysis and Western blotting of isolated splenocytes from Td/xid double heterozygotes rule out Tcfe3 as a likely candidate for the Td mutation.
ISSN:0938-8990
1432-1777
DOI:10.1007/BF00352419