Human Gene for β-Microseminoprotein: Its Promoter Structure and Chromosomal Localization

• Published in: Journal of biochemistry (Tokyo) 1995-02, Vol.117 (2), p.346-352
• Main Authors: Ochiai, Yukiko, Inazawa, Johji, Ueyama, Hisao, Ohkubo, Iwao
• Format: Article
• Language: English
• Subjects: Animals; Base Sequence; Carcinoma, Hepatocellular; Cell Line; Chloramphenicol O-Acetyltransferase - biosynthesis; chromosomal localization; Chromosome Mapping; Chromosomes, Human, Pair 10; DNA Primers; gene structure; Hominidae - genetics; human seminal plasma; Humans; In Situ Hybridization, Fluorescence; Liver Neoplasms; Male; Molecular Sequence Data; Plasmids; Polymerase Chain Reaction; Promoter Regions, Genetic; Prostatic Neoplasms; Prostatic Secretory Proteins; Protein Biosynthesis; Proteins - genetics; Recombinant Proteins - biosynthesis; Regulatory Sequences, Nucleic Acid; Restriction Mapping; Seminal Plasma Proteins; TATA Box; Testicular Hormones - genetics; Transfection; transient expression assay; Tumor Cells, Cultured; β-micro seminoprotein
• ISSN: 0021-924X
• DOI: 10.1093/jb/117.2.346

The gene for human β-microseminoprotein (MSP, β-inhibin, prostatic secretory protein94 or PSP94 was isolated. The nucleotide sequence of its upstream region (2,860 bp), exon 1(35 bp), and a part of the following intron (218 bp) was determined. Transient transfection analysis on MSP-expressing (PC-3) and -nonexpressing (HepG2) cells using luciferase reporter plasmids suggested that the region from -2738 to -276 is not essential for the basal promoter activity, and that the regions from -275 to -207 and from -186 to -128 function in a cell-specific manner. The chromosome locus of the gene (MSMB) was determined through application of PCR to the DNAs of rodent-human somatic cell hybrids and also by the fluorescence in situ hybridization technique to be region qll.2 of chromo some 10.