Identification of disulfide bonds in carboxy-terminal propeptides of human type I procollagen

Intra-chain and inter-chain disulfide bonds within the carboxy-terminal propeptides of human type I procollagen were studied using cyanogen bromide cleavage of the propeptides and electrophoresis on SDS-polyacrylamide/glycerol gels. The results could provide a better understanding of the assembly of...

Full description

Saved in:
Bibliographic Details
Published in:FEBS letters 1987-02, Vol.212 (2), p.229-232
Main Author: Koivu, Juha
Format: Article
Language:English
Subjects:
Analytical, structural and metabolic biochemistry
Biological and medical sciences
Carboxy-terminal propeptide
CNBr, cyanogen bromide
Cyanogen Bromide
Disulfide bond
Disulfides - analysis
DTT, dithiothreitol
Electrophoresis, Polyacrylamide Gel
Fundamental and applied biological sciences. Psychology
Glycoproteins
Humans
man
Peptide Fragments - analysis
Procollagen
Proteins
Type I procollagen
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Intra-chain and inter-chain disulfide bonds within the carboxy-terminal propeptides of human type I procollagen were studied using cyanogen bromide cleavage of the propeptides and electrophoresis on SDS-polyacrylamide/glycerol gels. The results could provide a better understanding of the assembly of proα-chains into procollagen.
ISSN:0014-5793
1873-3468
DOI:10.1016/0014-5793(87)81350-9