Myelin basic protein does not have a mitogenic effect on adult oligodendrocytes

Increased numbers of oligodendrocytes and remyelination are frequently observed in multiple sclerosis plaques. It is presumed the increased numbers of oligodendrocytes are due to cell division, but this has not been proven. The mitogens within the lesion which might be responsible for this are unkno...

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Bibliographic Details
Published in:Acta neuropathologica 1995-05, Vol.89 (5), p.431-437
Main Authors: MOORE, G. R. W, KIM, S. U, CHANG, E, KIM, M
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Bromodeoxyuridine - pharmacology
Cattle
Cell Division - drug effects
Cells, Cultured
Degenerative and inherited degenerative diseases of the nervous system. Leukodystrophies. Prion diseases
Electrophoresis, Polyacrylamide Gel
Fibrinolysin - metabolism
Galactosylceramides - metabolism
Immunohistochemistry
Medical sciences
Microscopy, Electron
Mitogens - pharmacology
Myelin Basic Protein - chemistry
Myelin Basic Protein - metabolism
Myelin Basic Protein - pharmacology
Neurology
Oligodendroglia - drug effects
Oligodendroglia - ultrastructure
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Summary:Increased numbers of oligodendrocytes and remyelination are frequently observed in multiple sclerosis plaques. It is presumed the increased numbers of oligodendrocytes are due to cell division, but this has not been proven. The mitogens within the lesion which might be responsible for this are unknown. Since oligodendrocyte proliferation occurs in areas in which there is myelin breakdown, we undertook the present study to determine if myelin basic protein (MBP) or its breakdown products could induce oligodendrocyte proliferation. MBP, or MBP digested by the neutral proteinase plasmin, was added in three concentrations to the media of adult bovine oligodendrocytes in culture. Oligodendrocytes were identified by staining for galactocerebroside. Bromodeoxyuridine incorporation was used as a measure of cell division. Oligodendrocytes were found to divide only rarely in regular culture media, in the presence of MBP, plasmin, or MBP digested by plasmin. The results indicate that MBP is not a significant mitogen for the mature oligodendrocyte.
ISSN:0001-6322
1432-0533
DOI:10.1007/BF00307648