Sequence analysis and comparison of ribosomal DNA from bovine Neospora to similar coccidial parasites

• Published in: The Journal of parasitology 1995-08, Vol.81 (4), p.530-535
• Main Authors: Marsh, A.E. (University of California, Davis, CA.), Barr, B.C, Sverlow, K, Ho, M, Dubey, J.P, Conrad, P.A
• Format: Article
• Language: English
• Subjects: ADN; Animals; ARN RIBOSOMAL; ARN RIBOSOMIAL; Base Sequence; Biological and medical sciences; BOVIN; CATTLE; Cattle Diseases - parasitology; Coccidia - classification; Coccidia - genetics; Coccidiosis - parasitology; Coccidiosis - veterinary; CRYPTOSPORIDIUM PARVUM; Cryptosporidium parvum - classification; Cryptosporidium parvum - genetics; DNA; DNA Primers - chemistry; DNA SEQUENCING; DNA, Protozoan - chemistry; DNA, Ribosomal - chemistry; Dog Diseases - parasitology; Dogs; Fundamental and applied biological sciences. Psychology; GANADO BOVINO; Gels; GENETIC VARIATION; Genetics-Evolution; Goats; Humans; Molecular Sequence Data; NEOSPORA; Neospora - classification; Neospora - genetics; NEOSPORA CANINUM; NUCLEOTIDE SEQUENCE; Nucleotide sequences; Nucleotides; Parasites; Parasitology; PHYLOGENETICS; Polymerase chain reaction; Protozoa; RIBOSOMAL RNA; RIBOSOMAS; RIBOSOME; RIBOSOMES; RNA, Protozoan - genetics; RNA, Small Nuclear - genetics; rRNA genes; Sarcocystis - classification; Sarcocystis - genetics; SARCOCYSTIS MURIS; SECUENCIA NUCLEOTIDICA; Sequence Alignment; SEQUENCE NUCLEOTIDIQUE; Sequencing; Systematics. Geographical distribution. Morphology. Cytology; TOXOPLASMA; Toxoplasma - classification; Toxoplasma - genetics; TOXOPLASMA GONDII; Ungulates; VARIACION GENETICA; VARIATION GENETIQUE
• ISSN: 0022-3395; 1937-2345
• DOI: 10.2307/3283848

The nuclear small subunit ribosomal RNA (nss-rRNA) gene sequence of Neospora spp. isolated from cattle was analyzed and compared to the sequences from several closely related cyst-forming coccidial parasites. Double-stranded DNA sequencing of 5 bovine Neospora spp. isolates (BPA1-4), 2 Neospora caninum isolates (NC-1 and NC-3), and 3 Toxoplasma gondii isolates (RH, GT-1, CT-1) were performed and compared to each other, as well as to other sequences available in GenBank for the NC-1 isolate, Sarcocystis muris, and Cryptosporidium parvum. There were no nucleotide differences detected between the Neospora spp. isolates from cattle and dogs. Four nucleotide differences were consistently detected when sequences of Neospora spp. isolates were compared to those of the T. gondii isolates. These results indicate that Neospora spp. and T. gondii are closely related, but distinct, species.