Structural and functional comparison of trematode haemoglobins

Haemoglobins from three different species of trematodes (Gastrothylax crumenifer, Paramphistomum epiclitum and Isoparorchis hypselobagri) and their respective hosts were digested with papain. Peptides so produced were separated on 10–15% SDS-PAGGE. The probability of coincidence of the peptides was...

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Bibliographic Details
Published in:Journal of helminthology 1995-03, Vol.69 (1), p.65-68
Main Authors: Rashid, K.A., Haque, M., Siddiqi, A.H.
Format: Article
Language:English
Subjects:
Animals
Biochemistry. Physiology. Immunology. Molecular biology
Biological and medical sciences
Fundamental and applied biological sciences. Psychology
Hemoglobins - chemistry
Hemoglobins - metabolism
Invertebrates
Kinetics
Nemathelminthia. Plathelmintha
Oxyhemoglobins - metabolism
Papain
Peptide Fragments - chemistry
Physiology. Development
Probability
Research Papers
Species Specificity
Trematoda - metabolism
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Summary:Haemoglobins from three different species of trematodes (Gastrothylax crumenifer, Paramphistomum epiclitum and Isoparorchis hypselobagri) and their respective hosts were digested with papain. Peptides so produced were separated on 10–15% SDS-PAGGE. The probability of coincidence of the peptides was analysed statistically. Oxygen affinity curves of trematode haemoglobins were found to be a rectangular hyperbolic. The Hill coefficient values of all the trematode haemoglobins were found to be 1. The P50 values, at 25°C in 0.2M phosphate buffer pH 7.4, ranged from 0.8 to 1.6 mmHg.
ISSN:0022-149X
1475-2697
DOI:10.1017/S0022149X00013857