The Immunoglobulin Octanucleotide: Independent Activity and Selective Interaction with Enhancers

The thymidine kinase (tk) promoter of herpes simplex virus includes an octanucleotide sequence motif (ATTTGCAT) that is also an essential component of immunoglobulin kappa gene promoters. In the absence of an enhancer, tk promoter derivatives that contain this element support a higher rate of transc...

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Bibliographic Details
Published in:Science (American Association for the Advancement of Science) 1987-03, Vol.235 (4795), p.1498-1501
Main Authors: Parslow, Tristram G., Jones, Susan Dana, Bond, Barbara, Yamamoto, Keith R.
Format: Article
Language:English
Subjects:
3T3 cells
Biochemistry
Biological and medical sciences
Cell lines
DNA
DNA, Viral - genetics
Enhancer Elements, Genetic
Enzymes
Fundamental and applied biological sciences. Psychology
Gene Expression Regulation
Genes
Genes, Regulator
Genetics
Herpes simplex virus
Immunoglobulin kappa-Chains - genetics
Immunoglobulins
Lymphocytes
Lymphocytes - physiology
Mammalia
Molecular and cellular biology
Molecular genetics
Moloney murine sarcoma virus - genetics
Plasmids
Promoter regions
Promoter Regions, Genetic
Promoters (Genetics)
Simplexvirus - genetics
Small nuclear RNA
Thymidine Kinase - genetics
Transcription. Transcription factor. Splicing. Rna processing
Viruses
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Summary:The thymidine kinase (tk) promoter of herpes simplex virus includes an octanucleotide sequence motif (ATTTGCAT) that is also an essential component of immunoglobulin kappa gene promoters. In the absence of an enhancer, tk promoter derivatives that contain this element support a higher rate of transcription than those that lack it. The action of the kappa enhancer augments that of the octanucleotide in B lymphoid cells; when both elements are present, tk promoter activity is increased by more than an order of magnitude. In contrast, the presence of the octanucleotide in this promoter markedly reduces its response to a nonimmunoglobulin enhancer. These results suggest that the octanucleotide may mediate a selective interaction among promoters and enhancers.
ISSN:0036-8075
1095-9203
DOI:10.1126/science.3029871