Effects of Antisense Oligonucleotides to the Cardiac Na+/Ca2+ Exchanger on Cultured Cardiac Myocytes

Antisense oligodeoxynucleotides based on the sequence of the cardiac Na+/Ca2+ exchanger were used to study the effects of decreasing expression of this protein on Na+-dependent Ca2+ uptake activity and on the spontaneous beating rate of cultured rat cardiac myocytes. Antisense oligos reduced Na+/Ca2...

Full description

Saved in:
Bibliographic Details
Published in:Biochemical and biophysical research communications 1995-07, Vol.212 (2), p.524-530
Main Authors: Takahashi, K., Bland, K.S., Islam, S., Michaelis, M.L.
Format: Article
Language:English
Subjects:
Animals
Base Sequence
Biological Transport - drug effects
Calcium - metabolism
Carrier Proteins - genetics
Carrier Proteins - physiology
Cells, Cultured
Gene Expression - drug effects
Heart - drug effects
Heart - embryology
Molecular Sequence Data
Myocardium - metabolism
Oligonucleotides, Antisense - pharmacology
Rats
Sodium - pharmacology
Sodium-Calcium Exchanger
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Antisense oligodeoxynucleotides based on the sequence of the cardiac Na+/Ca2+ exchanger were used to study the effects of decreasing expression of this protein on Na+-dependent Ca2+ uptake activity and on the spontaneous beating rate of cultured rat cardiac myocytes. Antisense oligos reduced Na+/Ca2+ exchanger activity of myocytes approximately 30-40%, compared to sense-treated or untreated controls. Antisense treatment also increased the spontaneous beating rate of myocardial cells from an average rate of 40 beats to 69 beats per minute, compared to sense-treated or untreated controls. These results indicate that reduced expression of the Na+/Ca2+ exchanger significantly alters intracellular Ca2+ regulation leading to an enhanced spontaneous beating rate and, presumably, a shortened duration of the cardiac cycle in developing myocytes.
ISSN:0006-291X
1090-2104
DOI:10.1006/bbrc.1995.2001