Topical application of calcitriol alters expression of filaggrin but not keratin K1 in mouse epidermis

Calcitriol (1 alpha,25-dihydroxyvitamin D3) and its analogues are antiproliferative agents which promote epidermal differentiation in vitro, possibly reflecting their modes of action in the treatment of psoriasis. We examined the effect of calcitriol on early and late terminal differentiation in mou...

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Published in:Archives of Dermatological Research 1995-05, Vol.287 (5), p.480-487
Main Authors: LÜTZOW-HOLM, C, HEYDEN, A, HUITFELDT, H. S, BRANDTZAEG, P, CLAUSEN, O. P. F
Format: Article
Language:English
Subjects:
Administration, Topical
Animals
Biological and medical sciences
Bromodeoxyuridine - metabolism
Calcitriol - pharmacology
Calcium - blood
Cell Differentiation - drug effects
Cell Division - drug effects
Epidermal Cells
Epidermis - drug effects
Epidermis - metabolism
Hormones. Endocrine system
Intermediate Filament Proteins - analysis
Keratinocytes - drug effects
Keratins - analysis
Medical sciences
Mice
Mice, Hairless
Pharmacology. Drug treatments
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Summary:Calcitriol (1 alpha,25-dihydroxyvitamin D3) and its analogues are antiproliferative agents which promote epidermal differentiation in vitro, possibly reflecting their modes of action in the treatment of psoriasis. We examined the effect of calcitriol on early and late terminal differentiation in mouse epidermis in vivo using an immunofluorescence assay to detect keratin K1 and filaggrin expression. Pulse labelling with the tymidine analogue 5-bromo-2-deoxyuridine (BrdUrd) was performed by intraperitoneal injection of mice immediately or 16 h after a single topical application of 0.72 nmol calcitriol. The BrdUrd labelling index (LI) and keratin K1 or filaggrin expression of postmitotic cell cohorts were scored by paired immunofluorescence staining for up to 72 h after BrdUrd labelling. Calcitriol induced cell proliferation as shown by a 100% increase in the BrdUrd LI 17 h after application. The onset of keratin K1 expression in the postmitotic period was, however, unchanged in both series after calcitriol treatment. Filaggrin expression appeared earlier after calcitriol treatment than in control epidermis, probably reflecting altered cell kinetics with increased epidermal turnover. The results suggest that calcitriol only influences the later stages of the keratinocyte differentiation programme, possibly secondarily to its hyperproliferative effect.
ISSN:0340-3696
1432-069X
DOI:10.1007/BF00373432