SgfI, a new type-II restriction endonuclease that recognizes the octanucleotide sequence 5′-GCGAT/CGC-3

A new restriction endonuclease (ENase), SgfI, has been isolated from the bacterium Streptomyces sp. SgfI recognizes the 8-bp palindrome 5′-GCGATCGC-3′ and cleaves double-stranded DNA after the T in this sequence, producing a two-base 3′ overhang compatible with PvuI termini. SgfI is a rare-cutting E...

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Bibliographic Details
Published in:Gene 1995-07, Vol.160 (1), p.55-58
Main Authors: Kappelman, James R., Brady, Melinda, Knoche, Kim, Murray, Elizabeth, Schoenfeld, Thomas, Williams, Ray, Vesselinova, Nadia
Format: Article
Language:English
Subjects:
Base Sequence
Cloning
CpG methylation
Deoxyribonucleases, Type II Site-Specific - isolation & purification
Deoxyribonucleases, Type II Site-Specific - metabolism
DNA - chemistry
DNA - metabolism
enzyme identification
genome analysis
Kinetics
Methylation
Molecular Sequence Data
nucleotide sequence
Oligodeoxyribonucleotides - metabolism
Streptomyces
Streptomyces - enzymology
Streptomyces sp
Substrate Specificity
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Summary:A new restriction endonuclease (ENase), SgfI, has been isolated from the bacterium Streptomyces sp. SgfI recognizes the 8-bp palindrome 5′-GCGATCGC-3′ and cleaves double-stranded DNA after the T in this sequence, producing a two-base 3′ overhang compatible with PvuI termini. SgfI is a rare-cutting ENase and should be useful for megabase mapping experiments.
ISSN:0378-1119
1879-0038
DOI:10.1016/0378-1119(95)00197-E