Strategies for the Synthesis and Screening of Glycoconjugates. 2. Covalent Immobilization for Flow Cytometry

Glycosylamines are readily available carbohydrate derivatives that undergo acylation reactions with homobifunctional N-hydroxysuccinimidyl esters. The product glycosylamides carry a spacer group equipped with one active ester functionality. This route provides well-defined glycoconjugates, which may...

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Bibliographic Details
Published in:Bioconjugate chemistry 1995-05, Vol.6 (3), p.319-322
Main Authors: Vetter, Dirk, Tate, Emily M, Gallop, Mark A
Format: Article
Language:English
Subjects:
Carbohydrate Sequence
Flow Cytometry
Fluorescent Dyes
Glycoconjugates - analysis
Glycoconjugates - chemical synthesis
Lectins - chemistry
Magnetic Resonance Spectroscopy
Molecular Sequence Data
Spectrometry, Mass, Fast Atom Bombardment
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Summary:Glycosylamines are readily available carbohydrate derivatives that undergo acylation reactions with homobifunctional N-hydroxysuccinimidyl esters. The product glycosylamides carry a spacer group equipped with one active ester functionality. This route provides well-defined glycoconjugates, which may be cross-linked to various amino-functionalized resins. Carbohydrate recognition of the resulting sugar-bead conjugates is probed by lectin immunostaining or flow cytometry using a fluorescently labeled lectin.
ISSN:1043-1802
1520-4812
DOI:10.1021/bc00033a014