Plasma 8‐epi‐PGF2α levels are elevated in individuals with non‐insulin dependent diabetes mellitus

This study reports plasma levels of a specific nonenzymatic peroxidation product of arachidonic acid, esterified 8‐epi‐PGF2α, from healthy‐ and NIDDM individuals as an index of oxidative stress in vivo. Plasma 8‐epi‐PGF2α was isolated by solid‐phase extraction on a C,8 followed by an NH2 cartridge a...

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Bibliographic Details
Published in:FEBS letters 1995-07, Vol.368 (2), p.225-229
Main Authors: Gopaul, N.K., Änggård, E.E., Mallet, A.I., Betteridge, D.J., Wolff, S.P., Nourooz-Zadeh, J.
Format: Article
Language:English
Subjects:
8-Epi-PGF2α
Adult
Aspirin - pharmacology
BHT
BSTFA
butylated hydroxytoluene
Chromatography, Gas - methods
Diabetes
Diabetes Mellitus, Type 2 - blood
diisopropylethylamine
Dinoprost - analogs & derivatives
Dinoprost - biosynthesis
Dinoprost - blood
DIPEA
Esterification
F2-isoprostane
gas chromatography-mass spectrometry/negative ion chemical ionisation
GC-MS/NICI
Humans
Indomethacin - pharmacology
Lipid peroxidation
Mass Spectrometry - methods
Models, Chemical
N, O-bis(trimethyl)-trifluoroacetamide
NIDDM
non-insulin-dependent diabetes mellitus
Oxidative Stress
pentafluorobenzyl
PFB
Prostaglandin
solid-phase extraction
SPE
TMS
trimethylsilyl
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Summary:This study reports plasma levels of a specific nonenzymatic peroxidation product of arachidonic acid, esterified 8‐epi‐PGF2α, from healthy‐ and NIDDM individuals as an index of oxidative stress in vivo. Plasma 8‐epi‐PGF2α was isolated by solid‐phase extraction on a C,8 followed by an NH2 cartridge and analyzed by GC‐MS/NICI as PFB‐ester/TMS‐ether derivative. We found that the average concentration of esterified 8‐epi‐PGF2α among NIDDM subjects (0.93 ± 0.07 nM, n = 39) was higher (P < 0.0001, Mann‐Whitney test) than in healthy individuals (0.28 ± 0.04 nM, n = 15). These data indicate that NIDDM is associated with increased plasma lipid peroxidation.
ISSN:0014-5793
1873-3468
DOI:10.1016/0014-5793(95)00649-T