Hepatic Function Is Preserved Following Liver-Directed, Adenovirus-Mediated Gene Transfer

• Published in: The Journal of surgical research 1995-08, Vol.59 (2), p.299-304
• Main Authors: Drazan, Kenneth E., Csete, Marie E., Shen, Xiu Da, Bullington, Deborah, Cottle, Gina, Busuttil, Ronald W., Shaked, Abraham
• Format: Article
• Language: English
• Subjects: Adenoviridae - genetics; Adenoviridae - physiology; Animals; Aspartate Aminotransferases - analysis; Aspartate Aminotransferases - blood; Base Sequence; beta-Galactosidase - genetics; Biological and medical sciences; Blotting, Western; Digestive system; Disease Models, Animal; DNA - analysis; DNA - chemistry; DNA - genetics; DNA Primers - chemistry; gamma-Glutamyltransferase - analysis; gamma-Glutamyltransferase - blood; Gene Transfer Techniques; Genetic Therapy - methods; Genetic Vectors; Investigative techniques, diagnostic techniques (general aspects); Liver - enzymology; Liver - pathology; Liver - physiology; Liver Transplantation; Male; Medical sciences; Molecular Sequence Data; Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques; Polymerase Chain Reaction; Promoter Regions, Genetic - genetics; Rats; Rats, Inbred Lew; RNA - analysis; RNA - chemistry; RNA - genetics
• ISSN: 0022-4804; 1095-8673
• DOI: 10.1006/jsre.1995.1168

Inherited and acquired disorders of the liver are attractive targets for gene therapy. Hepatic cells are susceptible targets for shuttle vectors because of a diversity of protein and viral receptors and accessibility of a selective afferent blood supply. Preservation of existing hepatic cell integrity and metabolic function is of paramount importance for successful whole animal gene therapy trials. In this report, we examine hepatic cell function and integrity following adenovirus-mediated reporter gene transfer to the liver in vivo. E1-deleted, replication-defective adenovectors encoding the LacZ gene driven by the human CMV promoter were delivered to the liver by isolated portal perfusion. The gene transfer rate, as determined by specific histochemical staining, approached 30% with recombinant protein detectable by Western blot throughout the course of study. Hepatic cell integrity as assessed by histology and hepatic enzyme profile (serum aspartate aminotransferase, γ-glutamyl transpeptidase) demonstrated normal cellular architecture and no significant difference between transfected liver and controls. Hepatic synthetic and metabolic function, as determined by albumin levels, prothrombin time, and bilirubin, were similar between the two study groups. This study demonstrates that efficient adenovirus-mediated gene transfer and expression in the rat liver do not compromise hepatic cell metabolism and integrity.