Identification of a gibberellin-induced gene in deepwater rice using differential display of mRNA

Differential display of mRNA was employed to identify gibberellin (GA)-regulated genes in deepwater rice. One of the first differentially displayed products identified was shown to be ten-fold induced after start of GA treatment. The sequence of the clone shows complete amino acid identity with hist...

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Bibliographic Details
Published in:Plant molecular biology 1995-06, Vol.28 (3), p.589-592
Main Authors: Knaap, E. van der (Michigan State Univ., East Lansing, MI (USA). Plant Research Lab.), Kende, H
Format: Article
Language:English
Subjects:
ACIDO GIBERELICO
Amino Acid Sequence
ARN MENSAJERO
ARN MESSAGER
Base Sequence
DNA, Plant
EXPRESION GENICA
EXPRESSION DES GENES
GENE EXPRESSION
Gene Expression Regulation, Plant - drug effects
Genes, Plant
GIBBERELLINE
Gibberellins - pharmacology
HISTONAS
HISTONE
HISTONES
MESSENGER RNA
Molecular Sequence Data
Oryza - genetics
ORYZA SATIVA
RNA, Messenger - genetics
Sequence Homology, Amino Acid
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Summary:Differential display of mRNA was employed to identify gibberellin (GA)-regulated genes in deepwater rice. One of the first differentially displayed products identified was shown to be ten-fold induced after start of GA treatment. The sequence of the clone shows complete amino acid identity with histone H3, and its increased mRNA level correlates with the onset of DNA synthesis. We also identified a gene whose expression pattern did not change over the course of treatment with GA and can be used as standard to correct for loading differences on northern blots.
ISSN:0167-4412
1573-5028
DOI:10.1007/bf00020405