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Identification of a gibberellin-induced gene in deepwater rice using differential display of mRNA

Differential display of mRNA was employed to identify gibberellin (GA)-regulated genes in deepwater rice. One of the first differentially displayed products identified was shown to be ten-fold induced after start of GA treatment. The sequence of the clone shows complete amino acid identity with hist...

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Published in:	Plant molecular biology 1995-06, Vol.28 (3), p.589-592
Main Authors:	Knaap, E. van der (Michigan State Univ., East Lansing, MI (USA). Plant Research Lab.), Kende, H
Format:	Article
Language:	English
Subjects:	ACIDO GIBERELICO Amino Acid Sequence ARN MENSAJERO ARN MESSAGER Base Sequence DNA, Plant EXPRESION GENICA EXPRESSION DES GENES GENE EXPRESSION Gene Expression Regulation, Plant - drug effects Genes, Plant GIBBERELLINE Gibberellins - pharmacology HISTONAS HISTONE HISTONES MESSENGER RNA Molecular Sequence Data Oryza - genetics ORYZA SATIVA RNA, Messenger - genetics Sequence Homology, Amino Acid
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creator	Knaap, E. van der (Michigan State Univ., East Lansing, MI (USA). Plant Research Lab.) Kende, H
description	Differential display of mRNA was employed to identify gibberellin (GA)-regulated genes in deepwater rice. One of the first differentially displayed products identified was shown to be ten-fold induced after start of GA treatment. The sequence of the clone shows complete amino acid identity with histone H3, and its increased mRNA level correlates with the onset of DNA synthesis. We also identified a gene whose expression pattern did not change over the course of treatment with GA and can be used as standard to correct for loading differences on northern blots.
doi_str_mv	10.1007/bf00020405
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language	eng
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source	Springer Online Journal Archives
subjects	ACIDO GIBERELICO Amino Acid Sequence ARN MENSAJERO ARN MESSAGER Base Sequence DNA, Plant EXPRESION GENICA EXPRESSION DES GENES GENE EXPRESSION Gene Expression Regulation, Plant - drug effects Genes, Plant GIBBERELLINE Gibberellins - pharmacology HISTONAS HISTONE HISTONES MESSENGER RNA Molecular Sequence Data Oryza - genetics ORYZA SATIVA RNA, Messenger - genetics Sequence Homology, Amino Acid
title	Identification of a gibberellin-induced gene in deepwater rice using differential display of mRNA
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