Comparison of Gamma-Ray-Induced Chromosome Ring and Inversion Frequencies

A method was used to detect chromosome inversions as apparent or false sister chromatid exchanges (SCEs) in the first mitosis after γ irradiation of human G0 cells. Dose-response relationships for small inversions have not been measured and reported previously, but it has been assumed that these are...

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Bibliographic Details
Published in:Radiation research 1995-08, Vol.143 (2), p.175-180
Main Authors: Mühlmann-Díaz, María C., Bedford, Joel S.
Format: Article
Language:English
Subjects:
Biological and medical sciences
Cell cycle
Cell Line
Cell physiology
CHO cells
Chromatids
Chromosome Inversion
Chromosomes
Cultured cells
Effects of physical and chemical agents
Fundamental and applied biological sciences. Psychology
Gamma Rays
Humans
Ionizing radiation
Irradiation
Lymphocytes
Mitosis
Molecular and cellular biology
Radiation dosage
Resting Phase, Cell Cycle - radiation effects
Ring Chromosomes
Sister Chromatid Exchange
Space life sciences
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Summary:A method was used to detect chromosome inversions as apparent or false sister chromatid exchanges (SCEs) in the first mitosis after γ irradiation of human G0 cells. Dose-response relationships for small inversions have not been measured and reported previously, but it has been assumed that these are induced with a frequency equal to that of their easily measured asymmetrical counterpart, the interstitial deletion. Our experiments confirm this expectation. The results also demonstrate, as others have suggested, that in protocols where SCEs have been reported in the first postirradiation mitosis after incorporation of BrdU in the previous cell cycle, the X- or γ-ray treatment of G0- or G1-phase cells produces virtually no true SCEs.
ISSN:0033-7587
1938-5404
DOI:10.2307/3579154