RNA fingerprinting and differential display using arbitrarily primed PCR

RNA fingerprinting by arbitrarily primed PCR can be used to detect and clone transcripts that are differentially expressed between cells that have been subject to different environments or developmental programs. The method also allows an estimate of the number of genes that are differentially expre...

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Bibliographic Details
Published in:Trends in Genetics 1995-06, Vol.11 (6), p.242-246
Main Authors: McClelland, Michael, Mathieu-Daude, Francoise, Welsh, John
Format: Article
Language:English
Subjects:
Animals
Base Sequence
Biological and medical sciences
Cloning, Molecular - methods
Diverse techniques
Fundamental and applied biological sciences. Psychology
Gene Expression Regulation
Genetic Techniques
Humans
Molecular and cellular biology
Molecular Sequence Data
Phenotype
Polymerase Chain Reaction - methods
RNA, Messenger - genetics
RNA, Messenger - isolation & purification
RNA-Directed DNA Polymerase - metabolism
Templates, Genetic
Transcription, Genetic
Citations: Items that cite this one
Online Access:Get full text
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Description
Summary:RNA fingerprinting by arbitrarily primed PCR can be used to detect and clone transcripts that are differentially expressed between cells that have been subject to different environments or developmental programs. The method also allows an estimate of the number of genes that are differentially expressed under various circumstances. When many experimental treatments are compared in parallel, intersecting regulatory pathways are reflected in genes that are perturbed by more than one treatment.
ISSN:0168-9525
DOI:10.1016/S0168-9525(00)89058-7