Differential in situ hybridization for herpes simplex virus typing in routine skin biopsies

A herpes simplex virus (HSV) type 2 specific recombinant plasmid probe designated pH 2S3 was constructed from non-HSV-1 crossreactive regions of the HSV-2 genome. DNA in situ hybridization on in vitro reconstructed tissue samples of sheep collagen matrix impregnated with herpes virus-infected human...

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Bibliographic Details
Published in:Journal of virological methods 1995-05, Vol.53 (1), p.37-45
Main Authors: Botma, Henk Jan, Dekker, Hetty, Van Amstel, Peter, Cairo, Irina, Van Den Berg, Frank M.
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Biopsy
Cattle
Cells, Cultured
Collagen matrix
Diagnosis
DNA Probes
DNA, Viral - analysis
Fundamental and applied biological sciences. Psychology
Herpes Simplex - diagnosis
Herpes Simplex - virology
Herpes simplex virus
Herpesvirus 1, Human - genetics
Herpesvirus 1, Human - isolation & purification
Herpesvirus 2, Human - genetics
Herpesvirus 2, Human - isolation & purification
Herpesvirus 3, Human - genetics
Herpesvirus 3, Human - isolation & purification
Humans
In situ hybridization
In Situ Hybridization - methods
Microbiology
Polyethylene Glycols
Skin - pathology
Skin - virology
Techniques used in virology
Tissue section
Type discrimination
Virology
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Summary:A herpes simplex virus (HSV) type 2 specific recombinant plasmid probe designated pH 2S3 was constructed from non-HSV-1 crossreactive regions of the HSV-2 genome. DNA in situ hybridization on in vitro reconstructed tissue samples of sheep collagen matrix impregnated with herpes virus-infected human cells was used to demonstrate absence of crossreactivity of the pH 2S3 probe with HSV-1 and varicella zoster virus under stringent posthybridization washing conditions. It was demonstrated that DNA in situ hybridization with pH 2S3 allows specific detection of HSV-2 in routine formalin-fixed, paraffin-embedded patient material.
ISSN:0166-0934
1879-0984
DOI:10.1016/0166-0934(94)00174-F