Pertussis toxin attenuates atrial natriuretic factor-mediated inhibition of adenylate cyclase. Involvement of inhibitory guanine nucleotide regulatory protein

The effect of pertussis toxin treatment was studied on the inhibitory effect of atrial natriuretic factor (ANF) on adenylate cyclase activity in rat aorta. The incubation of rat aorta washed particles with pertussis toxin and [alpha-32P]NAD resulted in the ADP-ribosylation of a single 40-kDa protein...

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Bibliographic Details
Published in:The Journal of biological chemistry 1987-04, Vol.262 (11), p.4931-4934
Main Authors: Anand-Srivastava, M.B., Srivastava, A.K., Cantin, M.
Format: Article
Language:English
Subjects:
adenylate cyclase
Adenylate Cyclase Toxin
Adenylyl Cyclase Inhibitors
Animals
aorta
Aorta - drug effects
Aorta - enzymology
atrial natriuretic factor
Atrial Natriuretic Factor - pharmacology
Biological and medical sciences
Bordetella pertussis
Cell physiology
Colforsin - pharmacology
Fundamental and applied biological sciences. Psychology
GTP-Binding Proteins - metabolism
Guanosine 5'-O-(3-Thiotriphosphate)
Guanosine Triphosphate - analogs & derivatives
Guanosine Triphosphate - pharmacology
Hormonal regulation
Isoproterenol - pharmacology
Male
Molecular and cellular biology
Molecular Weight
NAD - metabolism
Pertussis Toxin
Rats
Rats, Inbred Strains
Receptors, Atrial Natriuretic Factor
Receptors, Cell Surface - metabolism
Thionucleotides - pharmacology
toxins
Virulence Factors, Bordetella - pharmacology
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Summary:The effect of pertussis toxin treatment was studied on the inhibitory effect of atrial natriuretic factor (ANF) on adenylate cyclase activity in rat aorta. The incubation of rat aorta washed particles with pertussis toxin and [alpha-32P]NAD resulted in the ADP-ribosylation of a single 40-kDa protein. In addition, pertussis toxin treatment enhanced guanosine 5'-O-(thiotriphosphate) and isoproterenol-stimulated adenylate cyclase activities and attenuated the ANF-mediated inhibition of basal, isoproterenol-, and forskolin-stimulated adenylate cyclase activities. These data suggest that ANF receptors are coupled to adenylate cyclase through inhibitory guanine nucleotide regulatory protein.
ISSN:0021-9258
1083-351X
DOI:10.1016/S0021-9258(18)61132-9