Chronic ethanol consumption affects cholinoceptor- and purinoceptor-mediated contractions of the isolated rat bladder

• Published in: Alcohol (Fayetteville, N.Y.) N.Y.), 1995-05, Vol.12 (3), p.183-188
• Main Authors: Knight, G.E., Brizzolara, A.L., Soediono, P.O., Karoon, P., Burnstock, G.
• Format: Article
• Language: English
• Subjects: Adenosine Triphosphate - analogs & derivatives; Adenosine Triphosphate - pharmacology; Alcohol Drinking; Alcoholism and acute alcohol poisoning; Animals; Biological and medical sciences; Carbachol - pharmacology; Detrusor; Dose-Response Relationship, Drug; Electric Stimulation; Ethanol; In Vitro Techniques; Male; Medical sciences; Muscarinic; Muscle Contraction - drug effects; Osmolar Concentration; Purinergic; Rat; Rats; Rats, Sprague-Dawley; Receptors, Cholinergic - physiology; Receptors, Purinergic - physiology; Time Factors; Toxicology; Urinary Bladder - drug effects; Urinary Bladder - physiology
• ISSN: 0741-8329; 1873-6823
• DOI: 10.1016/0741-8329(94)00080-W

Isolated bladder strips from 12-week ethanol-fed, pair-fed, and control adult male rats were investigated. Contractile responses to carbachol (CCh; 0.1–300 μM) were statistically significantly potentiated in the ethanol-fed group compared to pair-fed and control. Contractions to β,γ-methylene ATP (β,γ-McATP; 1–300μM) were statistically significantly potentiated in the ethanol-fed group at the highest concentration tested (300 μM). Neurogenic contractions (0.5–32 pps) from the ethanol-fed group in the absence of atropine and after desensitisation by α,β-methylene ATP (α,β-McATP; 3 μM), were significantly potentiated compared to the pair-fed and control groups; in the presence of atropine (1 μM), neurogenic contractions were significantly augmented at the higher frequencies. It is concluded that chronic ethanol treatment affects both cholinoceptor- and purinoceptor-mediated contractions of the rat bladder.