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Changes in Shape and Motility of Cells Transfected with Parvalbumin cDNA
Parvalbumin is thought to act as a Ca 2+ buffer in skeletal muscle fibers, but its physiological role in brain, kidney, and testis remains unclear. We have transfected parvalbumin cDNA into a human ovarian adenocarcinoma cell line, which normally does not express this protein. The induced expression...
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| Published in: | Experimental cell research 1995-08, Vol.219 (2), p.420-426 |
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| Main Authors: | , , , , , , |
| Format: | Article |
| Language: | English |
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| cited_by | cdi_FETCH-LOGICAL-c271t-774b8cb32c73ac8356edff61f77ee29e187cb90ef513503d860f9db33340aa373 |
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| container_end_page | 426 |
| container_issue | 2 |
| container_start_page | 420 |
| container_title | Experimental cell research |
| container_volume | 219 |
| creator | Andressen, Christian Gotzos, Vassilis Berchtold, Martin W. Pauls, Thomas L. Schwaller, Beat Fellay, Benoit Celio, Marco R. |
| description | Parvalbumin is thought to act as a Ca
2+ buffer in skeletal muscle fibers, but its physiological role in brain, kidney, and testis remains unclear. We have transfected parvalbumin cDNA into a human ovarian adenocarcinoma cell line, which normally does not express this protein. The induced expression of parvalbumin under the control of three different promoters causes: (1) changes in the morphology of the cells from epitheloid to fusiform, (2) an increase in motility of whole cell clusters, and (3) a decrease in the mitotic rate. Transfection with a mutated cDNA of rat parvalbumin incapable of binding Ca
2+ had no effect on these three parameters. Our results indicate that ectopic expression of parvalbumin influences not only cell division [Rasmussen and Means (1989)
Mol. Endocrinol. 3, 588-596], but also cell shape and motility by modulating intracellular Ca
2+ handling. This may be a basic function of parvalbumin when it is intrinsically expressed in differentiated nonmuscle cells. |
| doi_str_mv | 10.1006/excr.1995.1248 |
| format | article |
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2+ had no effect on these three parameters. Our results indicate that ectopic expression of parvalbumin influences not only cell division [Rasmussen and Means (1989)
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2+ had no effect on these three parameters. Our results indicate that ectopic expression of parvalbumin influences not only cell division [Rasmussen and Means (1989)
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2+ buffer in skeletal muscle fibers, but its physiological role in brain, kidney, and testis remains unclear. We have transfected parvalbumin cDNA into a human ovarian adenocarcinoma cell line, which normally does not express this protein. The induced expression of parvalbumin under the control of three different promoters causes: (1) changes in the morphology of the cells from epitheloid to fusiform, (2) an increase in motility of whole cell clusters, and (3) a decrease in the mitotic rate. Transfection with a mutated cDNA of rat parvalbumin incapable of binding Ca
2+ had no effect on these three parameters. Our results indicate that ectopic expression of parvalbumin influences not only cell division [Rasmussen and Means (1989)
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2+ handling. This may be a basic function of parvalbumin when it is intrinsically expressed in differentiated nonmuscle cells.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>7641793</pmid><doi>10.1006/excr.1995.1248</doi><tpages>7</tpages></addata></record> |
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| ispartof | Experimental cell research, 1995-08, Vol.219 (2), p.420-426 |
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| language | eng |
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| source | ScienceDirect Freedom Collection 2022-2024 |
| subjects | Cell Line, Transformed Cell Movement Cell Size DNA, Complementary - genetics Female Gene Transfer Techniques Humans Ovary - cytology Ovary - metabolism Parvalbumins - genetics Parvalbumins - metabolism |
| title | Changes in Shape and Motility of Cells Transfected with Parvalbumin cDNA |
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