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Sarcolemmal Chloride and Potassium Channels from Normal and Myotonic Mouse Muscle Studied in Lipid-Supplemented Vesicles

By patch-clamp analysis of lipid supplemented vesicles prepared from the sarcolemma of mouse wildtype skeletal muscle, we could identify two known types of potassium channels, the inward rectifier and Ca2+ activated BK channels, and five types of chloride channels designated CIC-a, CIC-b, CIC-c, CIC...

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Bibliographic Details
Published in:Biochemical and biophysical research communications 1995-08, Vol.213 (2), p.513-518
Main Authors: Wischmeyer, E., Weberschurholz, S., Jockusch, H.
Format: Article
Language:English
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Summary:By patch-clamp analysis of lipid supplemented vesicles prepared from the sarcolemma of mouse wildtype skeletal muscle, we could identify two known types of potassium channels, the inward rectifier and Ca2+ activated BK channels, and five types of chloride channels designated CIC-a, CIC-b, CIC-c, CIC-d, and CIC-e. CIC-b corresponds to a known chloride channel, whereas CICa, -c, -d and -e have not been described previously. The diversity may be due to a heterooligomeric composition of different subunits. None of these chloride channels nor the potassium channels were found in the sarcoplasmic reticulum fraction. In vesicles from myotonic mice, Clc-d and -e were not found.
ISSN:0006-291X
1090-2104
DOI:10.1006/bbrc.1995.2161