Newcastle disease virus V protein binds zinc

The V protein of Newcastle disease virus (NDV) is produced by the insertion of a single nontemplated G residue at a specific point during transcription of the phosphoprotein (P) gene, accessing a new reading frame upon translation. The V protein, in common with its counterpart in other paramyxovirus...

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Bibliographic Details
Published in:Archives of virology 1995-07, Vol.140 (7), p.1321-1328
Main Authors: Steward, M, Samson, A.C.R, Errington, W, Emmerson, P.T
Format: Article
Language:English
Subjects:
Animals
Base Sequence
binding proteins
Biological and medical sciences
cysteine
Fundamental and applied biological sciences. Psychology
Microbiology
Molecular Sequence Data
Morphology, structure, chemical composition, physicochemical properties
Newcastle disease virus
Oligodeoxyribonucleotides
Phosphoproteins - genetics
Phosphoproteins - metabolism
plasmids
Protein Binding
Recombinant Fusion Proteins - metabolism
translation
viral proteins
Viral Proteins - genetics
Viral Proteins - isolation & purification
Viral Proteins - metabolism
Virology
zinc
Zinc - metabolism
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Summary:The V protein of Newcastle disease virus (NDV) is produced by the insertion of a single nontemplated G residue at a specific point during transcription of the phosphoprotein (P) gene, accessing a new reading frame upon translation. The V protein, in common with its counterpart in other paramyxoviruses contains a highly cysteine rich motif near the carboxyl terminus, suggestive of a zinc-binding domain. By constructing E. coli overexpression plasmids for the NDV P and V proteins, and monitoring the binding of 65ZnCl2 to proteins electroblotted onto nitrocellulose membranes, we have demonstrated that the V protein strongly binds zinc.
ISSN:0304-8608
1432-8798
DOI:10.1007/bf01322759