Ubiquitination of full-length cyclin

Mitotic cyclins are key cell-cycle regulators that are relatively stable through most of the cell-cycle then rapidly degraded at mitosis. We have detected ubiquitin conjugates of full-length Xenopus cyclin B2 strongly suggesting that ubiquitination rather than a proteolytic cleavage is the initiatin...

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Bibliographic Details
Published in:FEBS letters 1995-08, Vol.370 (1), p.109-112
Main Authors: Mahaffey, David T., Yung, Yoo, Rechsteiner, Martin
Format: Article
Language:English
Subjects:
Animals
Calcium - pharmacology
Cell Cycle
Cyclin B2
Cyclins - biosynthesis
Cyclins - isolation & purification
Cyclins - metabolism
Electrophoresis, Polyacrylamide Gel
Female
In Vitro Techniques
Interphase
Methylation
Oocytes - cytology
Oocytes - drug effects
Oocytes - metabolism
Protein Biosynthesis
Protein Processing, Post-Translational
RNA, Messenger - analysis
RNA, Messenger - biosynthesis
Ubiquitin
Ubiquitins - metabolism
Xenopus laevis
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Summary:Mitotic cyclins are key cell-cycle regulators that are relatively stable through most of the cell-cycle then rapidly degraded at mitosis. We have detected ubiquitin conjugates of full-length Xenopus cyclin B2 strongly suggesting that ubiquitination rather than a proteolytic cleavage is the initiating event in cyclin destruction. The highest levels of ubiquitin conjugates correlate with the phase of rapid proteolysis. This result supports previous findings that implicate the ubiquitin system in cyclin proteolysis. However, we also observe cyclin-ubiquitin conjugates in both cytostatic factor arrested and interphase extracts where cyclin is more stable. The physiologic role of ubiquitinated cyclin under these conditions is nuclear.
ISSN:0014-5793
1873-3468
DOI:10.1016/0014-5793(95)00799-F