Effects of fibroblasts of different origin on long term maintenance of xenotransplanted human epidermal keratinocytes in immunodeficient mice

We examined effects of fibroblasts of different origin on long-term maintenance of xenotransplanted human epidermal keratinocytes. A suspension of cultured epidermal cells, originating from adult human trunk skin, was injected into double mutant immunodeficient (BALB/c nu/scid) mice subcutaneously,...

Full description

Saved in:
Bibliographic Details
Published in:Cell and tissue research 1995-08, Vol.281 (2), p.223-229
Main Authors: Inokuchi, S, Shimamura, K, Tohya, H, Kidokoro, M, Tanaka, M, Ueyama, Y, Sawada, Y
Format: Article
Language:English
Subjects:
Animals
Basement Membrane - metabolism
Bone Marrow Cells
Cells, Cultured
Collagen - metabolism
Epidermal Cells
Epidermal Cyst
Fibroblasts - physiology
Graft Survival
Humans
Keratinocytes - transplantation
Laminin - metabolism
Membrane Proteins - metabolism
Mice
Mice, Inbred BALB C
Mice, Nude
Mice, SCID
Transplantation, Heterologous
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:We examined effects of fibroblasts of different origin on long-term maintenance of xenotransplanted human epidermal keratinocytes. A suspension of cultured epidermal cells, originating from adult human trunk skin, was injected into double mutant immunodeficient (BALB/c nu/scid) mice subcutaneously, with or without cultured fibroblastic cells of different origin. At one week after transplantation, the epidermal cells generated epidermoid cysts consisting of human epidermis-like tissue. When the epidermal cells were injected alone or together with fibroblastic cells derived from human bone marrow, muscle fascia, or murine dermis, organized epidermoid cysts regressed within 6 weeks. In contrast, when the epidermal cells were injected together with human dermal fibroblasts, generated epidermoid cysts were maintained in vivo for more than 24 weeks. Histological examination showed that the reorganized epidermis, after injection of both epidermal keratinocytes and dermal fibroblasts, retained normal structures of the original epidermis during 6 to 24 weeks after transplantation. The results indicate that human dermal fibroblasts facilitate the long-term maintenance of the reorganized epidermis after xenotransplantation of cultured human epidermal keratinocytes by supporting self renewal of the human epidermal tissue in vivo.
ISSN:0302-766X
DOI:10.1007/s004410050419