Transport and storage of Helicobacter pylori from gastric mucosal biopsies and clinical isolates

Various transport and storage conditions for the recovery of Helicobacter pylori from gastric biopsies were evaluated. Gastric mucosal biopsies from 16 Helicobacter pylori-infected patients were stored in cysteine-Albimi medium containing 20% glycerol in a refrigerator (4 degrees C) for 1 and 2 week...

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Bibliographic Details
Published in:European journal of clinical microbiology & infectious diseases 1995-04, Vol.14 (4), p.349-352
Main Authors: HAN, S. W, FLAMM, R, HACHEM, C. Y, KIM, H. Y, CLARRIDGE, J. E, EVANS, D. G, BEYER, J, DRNEC, J, GRAHAM, D. Y
Format: Article
Language:English
Subjects:
Bacterial diseases
Bacterial diseases of the digestive system and abdomen
Biological and medical sciences
Biopsy
Colony Count, Microbial
Culture Media
Gastric Mucosa - microbiology
Gastric Mucosa - pathology
Helicobacter Infections - pathology
Helicobacter pylori
Helicobacter pylori - growth & development
Helicobacter pylori - isolation & purification
Human bacterial diseases
Humans
Infectious diseases
Male
Medical sciences
Peptic Ulcer - microbiology
Peptic Ulcer - pathology
Specimen Handling
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Summary:Various transport and storage conditions for the recovery of Helicobacter pylori from gastric biopsies were evaluated. Gastric mucosal biopsies from 16 Helicobacter pylori-infected patients were stored in cysteine-Albimi medium containing 20% glycerol in a refrigerator (4 degrees C) for 1 and 2 weeks and in a -20 degrees C laboratory freezer for 4 and 12 weeks. Two clinical isolates were stored in saline, Stuart's transport media, cysteine-Albimi broth with 20% glycerol, brucella broth with 20% glycerol and skim milk with 17% glycerol at room temperature, 4 degrees C, -20 degrees C and -70 degrees C. Storage at 4 degrees C for 1 and 2 weeks resulted in Helicobacter pylori recovery from 81% and 19% of biopsies, respectively. Storage at -20 degrees C yielded Helicobacter pylori recovery in 100% and 57% after 4 and 12 weeks, respectively. At room temperature after 6 h, the Helicobacter pylori titer was reduced. The best storage media for frozen isolates were skim milk/glycerol, brucella broth/glycerol and cysteine-Albimi/glycerol (in descending order). Recovery was better at -70 degrees C than -20 degrees C.
ISSN:0934-9723
1435-4373
DOI:10.1007/BF02116531