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Herpesvirus of Turkey Recombinant Viruses Expressing Infectious Bursal Disease Virus (IBDV) VP2 Immunogen Induce Protection against an IBDV Virulent Challenge in Chickens

Two recombinant herpesviruses of turkey (HVT) expressing the VP2 protein of infectious bursal disease virus (IBDV or Gumboro disease virus) have been constructed vHVT001 and vHVT002. The VP2 open reading frame was inserted at the locus of the small subunit of ribonucleotide reductase gene (HSV-1 UL4...

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Published in:Virology (New York, N.Y.) N.Y.), 1995-08, Vol.211 (2), p.481-490
Main Authors: Darteil, Raphaël, Bublot, Michel, Laplace, Eliane, Bouquet, Jean-François, Audonnet, Jean-Christophe, Rivière, Michel
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container_title Virology (New York, N.Y.)
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Bublot, Michel
Laplace, Eliane
Bouquet, Jean-François
Audonnet, Jean-Christophe
Rivière, Michel
description Two recombinant herpesviruses of turkey (HVT) expressing the VP2 protein of infectious bursal disease virus (IBDV or Gumboro disease virus) have been constructed vHVT001 and vHVT002. The VP2 open reading frame was inserted at the locus of the small subunit of ribonucleotide reductase gene (HSV-1 UL40 homolog) without any exogenous promoter in vHVT001 and at the locus of gl gene (HSV-1 US7 homolog) under the control of the human cytomegalovirus immediate-early promoter in vHVT002. The isolation of these recombinant viruses indicated that the deleted genes were not required for replication of HVT in chicken embryo fibroblasts. Efficacy of these recombinant viruses against IBDV strain 52/70 and Marek's disease virus (MDV strain RB1B) virulent challenges was evaluated in chickens vaccinated at 1 day of age. In the IBDV challenge, a good protection against mortality and bursal gross lesion was observed in vHVT002-vaccinated chickens: 100% with 105 PFU dose and 60% with 104 PFU dose; in contrast, only a weak level of protection was achieved after vaccination with vHVT001. Protection levels against MDV challenge obtained with vHVT001 and vHVTO02 were low (around 10%) compared to that induced by the parental HVT (84%). In spite of the low protection level against MDV, this is the first report which describes induction of full protection against IBDV with a single inoculation of a recombinant virus.
doi_str_mv 10.1006/viro.1995.1430
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Protection levels against MDV challenge obtained with vHVT001 and vHVTO02 were low (around 10%) compared to that induced by the parental HVT (84%). 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control</topic><topic>Molecular Sequence Data</topic><topic>pathogenicity</topic><topic>poder patogeno</topic><topic>pollo</topic><topic>poulet</topic><topic>pouvoir pathogene</topic><topic>Promoter Regions, Genetic</topic><topic>proteinas</topic><topic>proteine</topic><topic>proteins</topic><topic>synthetic vaccines</topic><topic>Transfection</topic><topic>Turkey</topic><topic>vaccin synthetique</topic><topic>vaccination</topic><topic>Vaccines, Synthetic - therapeutic use</topic><topic>vacuna sintetica</topic><topic>vacunacion</topic><topic>Viral Structural Proteins - genetics</topic><topic>Viral Structural Proteins - immunology</topic><topic>Virulence</topic><topic>virus de los animales</topic><topic>virus des animaux</topic><topic>Virus Replication</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Darteil, Raphaël</creatorcontrib><creatorcontrib>Bublot, Michel</creatorcontrib><creatorcontrib>Laplace, Eliane</creatorcontrib><creatorcontrib>Bouquet, Jean-François</creatorcontrib><creatorcontrib>Audonnet, Jean-Christophe</creatorcontrib><creatorcontrib>Rivière, Michel</creatorcontrib><creatorcontrib>Laboratoire de Lyon, Lyon, France</creatorcontrib><creatorcontrib>South African Poultry Association, Honeydew (South Africa)</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Virology (New York, N.Y.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Darteil, Raphaël</au><au>Bublot, Michel</au><au>Laplace, Eliane</au><au>Bouquet, Jean-François</au><au>Audonnet, Jean-Christophe</au><au>Rivière, Michel</au><aucorp>Laboratoire de Lyon, Lyon, France</aucorp><aucorp>South African Poultry Association, Honeydew (South Africa)</aucorp><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Herpesvirus of Turkey Recombinant Viruses Expressing Infectious Bursal Disease Virus (IBDV) VP2 Immunogen Induce Protection against an IBDV Virulent Challenge in Chickens</atitle><jtitle>Virology (New York, N.Y.)</jtitle><addtitle>Virology</addtitle><date>1995-08-20</date><risdate>1995</risdate><volume>211</volume><issue>2</issue><spage>481</spage><epage>490</epage><pages>481-490</pages><issn>0042-6822</issn><eissn>1096-0341</eissn><abstract>Two recombinant herpesviruses of turkey (HVT) expressing the VP2 protein of infectious bursal disease virus (IBDV or Gumboro disease virus) have been constructed vHVT001 and vHVT002. 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Protection levels against MDV challenge obtained with vHVT001 and vHVTO02 were low (around 10%) compared to that induced by the parental HVT (84%). In spite of the low protection level against MDV, this is the first report which describes induction of full protection against IBDV with a single inoculation of a recombinant virus.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>7645252</pmid><doi>10.1006/viro.1995.1430</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record>
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identifier ISSN: 0042-6822
ispartof Virology (New York, N.Y.), 1995-08, Vol.211 (2), p.481-490
issn 0042-6822
1096-0341
language eng
recordid cdi_proquest_miscellaneous_77463521
source Elsevier
subjects animal viruses
Animals
antigene
antigenos
antigens
avian infectious bursitis
Base Sequence
Birnaviridae Infections - immunology
Birnaviridae Infections - prevention & control
Chick Embryo
Chickens
enfermedad de gumboro
Herpesviridae - genetics
Herpesviridae - immunology
herpetoviridae
infectious bursal disease virus
Infectious bursal disease virus - genetics
Infectious bursal disease virus - pathogenicity
Infectious bursal disease virus - physiology
maladie de gumboro
Marek Disease - immunology
Marek Disease - prevention & control
Molecular Sequence Data
pathogenicity
poder patogeno
pollo
poulet
pouvoir pathogene
Promoter Regions, Genetic
proteinas
proteine
proteins
synthetic vaccines
Transfection
Turkey
vaccin synthetique
vaccination
Vaccines, Synthetic - therapeutic use
vacuna sintetica
vacunacion
Viral Structural Proteins - genetics
Viral Structural Proteins - immunology
Virulence
virus de los animales
virus des animaux
Virus Replication
title Herpesvirus of Turkey Recombinant Viruses Expressing Infectious Bursal Disease Virus (IBDV) VP2 Immunogen Induce Protection against an IBDV Virulent Challenge in Chickens
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