Identification of Two Human Sequence-Specific DNA-Binding Proteins which Interact with the Direct Repeats in the Squirrel Monkey Retrovirus Long Terminal Repeat

Squirrel monkey retrovirus (SMRV) is a xenotropic endogenous virus of the squirrel monkey. The long terminal repeat of the SMRV contains four direct repeats (43 or 42 bp) with different degrees of perfection in the U3 region. Herein, we found two human nuclear proteins (SMBP1 and SMBP2) which bind s...

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Bibliographic Details
Published in:Virology (New York, N.Y.) N.Y.), 1995-08, Vol.211 (1), p.316-319
Main Authors: Ikeda, Shogo, Watanabe, Tsunakazu, Oda, Takuzo
Format: Article
Language:English
Subjects:
Animals
Base Sequence
Binding Sites
Cell Line
Cell Nucleus - metabolism
Chromatography, Ion Exchange
DNA, Viral - chemistry
DNA, Viral - metabolism
DNA-Binding Proteins - isolation & purification
DNA-Binding Proteins - metabolism
Humans
Molecular Sequence Data
Molecular Weight
Nuclear Proteins - isolation & purification
Nuclear Proteins - metabolism
Promoter Regions, Genetic
Repetitive Sequences, Nucleic Acid
Retroviridae - genetics
Retroviridae - isolation & purification
Saimiri - virology
TATA Box
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Summary:Squirrel monkey retrovirus (SMRV) is a xenotropic endogenous virus of the squirrel monkey. The long terminal repeat of the SMRV contains four direct repeats (43 or 42 bp) with different degrees of perfection in the U3 region. Herein, we found two human nuclear proteins (SMBP1 and SMBP2) which bind specifically to the direct repeats with different affinities The binding proteins recognize overlapped sequences in the 5′ region of the repeats. SMBP1 and 2 are monomeric proteins of 35 and 17 kDa, respectively.
ISSN:0042-6822
1096-0341
DOI:10.1006/viro.1995.1408