Distribution of individual cytoplasmic pH values in a population of the yeast Saccharomyces cerevisiae

Fluorescence ratio imaging microscopy using pH‐sensitive fluorescent dyes makes it possible to evaluate statistical distribution of intracellular pH in a population of the yeast S. cerevisiae examined in a thin layer of suspension in a Petri dish. The distribution appears to fit a Gaussian curve wit...

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Bibliographic Details
Published in:FEMS microbiology letters 1995-08, Vol.130 (2‐3), p.245-251
Main Authors: Cimprich, Petr, Slavík, Jan, Kotyk, Arnošt
Format: Article
Language:English
Subjects:
Biological and medical sciences
Cytoplasm - metabolism
Fluorescent Dyes
Fluorescent probe
Fundamental and applied biological sciences. Psychology
Growth, nutrition, metabolism, transports, enzymes. Molecular biology
Hydrogen-Ion Concentration
Intracellular pH
Microbiology
Mycology
Ratio imaging
Saccharomyces cerevisiae
Saccharomyces cerevisiae - metabolism
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Summary:Fluorescence ratio imaging microscopy using pH‐sensitive fluorescent dyes makes it possible to evaluate statistical distribution of intracellular pH in a population of the yeast S. cerevisiae examined in a thin layer of suspension in a Petri dish. The distribution appears to fit a Gaussian curve with a half‐width around the 0.4 pH unit. The curve became slightly narrower after resuspension in a strong buffer; the mean values shifted with the pH of the buffer. The shape of the distribution curves of both resting and growing cells in various phases of growth does not change significantly. Likewise, addition of 1% of glucose, 50 μM suloctidil or 100 μM diethylstilbestrol brings about no alteration. The only value which clearly changes is the average cytoplasmic pH.
ISSN:0378-1097
1574-6968
DOI:10.1111/j.1574-6968.1995.tb07727.x