Molecular cloning of two tandemly arranged peroxidase genes from Populus kitakamiensis and their differential regulation in the stem

A genomic library was prepared from Populus kitakamiensis and screened with the cDNA for an anionic peroxidase from P. kitakamiensis. One genomic clone was isolated that contained two tandemly oriented genes for anionic peroxidases, prxA3a and prxA4a. Both genes consisted of four exons and three int...

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Bibliographic Details
Published in:Plant molecular biology 1995-07, Vol.28 (4), p.677-689
Main Authors: Osakabe, K. (Tokyo Univ. of Agriculture and Technology, Fuchu, Tokyo (Japan). Faculty of Agriculture, Lab. of Wood Chemistry), Koyama, H, Kawai, S, Katayama, Y, Morohoshi, N
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Base Sequence
Blotting, Northern
Blotting, Southern
CLONACION MOLECULAR
CLONAGE MOLECULAIRE
EXPRESION GENICA
EXPRESSION DES GENES
Gene Dosage
GENE EXPRESSION
Genomic Library
Isoenzymes - biosynthesis
Isoenzymes - genetics
Lignin - biosynthesis
MOLECULAR CLONING
Molecular Sequence Data
Multigene Family - genetics
PEROXIDASAS
PEROXIDASES
Peroxidases - biosynthesis
Peroxidases - genetics
PEROXYDASE
Plant Stems - enzymology
POPULUS
Promoter Regions, Genetic - genetics
Sequence Analysis, DNA
Sequence Homology, Amino Acid
Transcription, Genetic
Trees - genetics
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Summary:A genomic library was prepared from Populus kitakamiensis and screened with the cDNA for an anionic peroxidase from P. kitakamiensis. One genomic clone was isolated that contained two tandemly oriented genes for anionic peroxidases, prxA3a and prxA4a. Both genes consisted of four exons and three introns; the introns had consensus nucleotides, namely, GT and AG, at their 5' and 3' ends, respectively. The prxA3a and prxA4a genes encoded 347 and 343 amino acid residues, respectively, including putative signal sequences at the amino-termini. Putative promoters and polyadenylation signals were found in the flanking regions of both genes. The sequence of the coding region of prxA3a was completely identical to that of the cDNA clone pA3, whereas the sequence of the coding region of prxA4a was only 73% identical to that of the cDNA clone pA3. Northern blot analysis showed that the patterns of expression of the mRNAs that corresponded to prxA3a and prxA4a differed in stems of P. kitakamiensis.
ISSN:0167-4412
1573-5028
DOI:10.1007/BF00021193