Efficacy of Murine Malaria Sporozoite Vaccines: Implications for Human Vaccine Development

As part of a study of potential vaccines against malaria, the protective efficacy of sporozoite subunit vaccines was determined by using the Plasmodium berghei murine malaria model. Mice were immunized with recombinant DNA-produced or synthetic peptide-carrier subunit vaccines derived from the repet...

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Bibliographic Details
Published in:Science (American Association for the Advancement of Science) 1987-04, Vol.236 (4800), p.453-456
Main Authors: Egan, James E., Weber, James L., Ballou, W. Ripley, Hollingdale, Michael R., Majarian, William R., Gordon, Daniel M., Maloy, W. Lee, Hoffman, Stephen L., Wirtz, Robert A., Schneider, Imogene, Woollett, Gillian R., Young, James F., Hockmeyer, Wayne T.
Format: Article
Language:English
Subjects:
Animals
Antibodies
Antigens
Antigens, Protozoan
Antigens, Surface - immunology
Biochemistry
Biological and medical sciences
Cellular immunity
Dose-Response Relationship, Immunologic
Epitopes
Experimental protozoal diseases and models
House mouse
Immunity, Cellular
Immunization
Immunization, Passive
Infectious diseases
Malaria
Malaria - prevention & control
Medical research
Medical sciences
Mice
Mice as laboratory animals
Oligopeptides - immunology
Parasitic diseases
Plasmodium berghei
Plasmodium berghei - immunology
Prevention
Protozoal diseases
Protozoan Proteins
Recombinant Fusion Proteins - immunology
Spleen cells
Sporozoites
Subunit vaccines
Vaccination
Vaccines
Vaccines, Synthetic
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Summary:As part of a study of potential vaccines against malaria, the protective efficacy of sporozoite subunit vaccines was determined by using the Plasmodium berghei murine malaria model. Mice were immunized with recombinant DNA-produced or synthetic peptide-carrier subunit vaccines derived from the repetitive epitopes of the Plasmodium berghei circumsporozoite gene, or with radiation-attenuated sporozoites. Immunization with subunit vaccines elicited humoral responses that were equivalent to or greater than those elicited by irradiated sporozoites, yet the protection against sporozoite challenge induced by either of the subunit vaccines was far less than that achieved by immunization with attenuated sporozoites. Passive and adoptive transfer studies demonstrated that subunit vaccines elicited predominantly antibody-mediated protection that was easily overcome whereas irradiated sporozoites induced potent cell-mediated immunity that protected against high challenge doses of sporozoites. These studies indicate that new strategies designed to induce cellular immunity will be required for efficacious sporozoite vaccines.
ISSN:0036-8075
1095-9203
DOI:10.1126/science.3551073