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Action of Carbon Tetrachloride in the Reconstituted Monooxygenase System Using Partially Purified Cytochrome P-450 and P-448

In the cytochrome P-450-reconstituted system, CCI4 stimulated NADPH-dependent lipid peroxidation of the system containing the P-450 form to a much greater extent than that of the system containing the P-448 form. When the P-450-reconstituted system was preincubated in the presence of both NADPH and...

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Published in:	Japanese Journal of Pharmacology 1987, Vol.43 (2), p.226-229
Main Authors:	MASUDA, Yasusuke, YASOSHIMA, Mitsue
Format:	Article
Language:	English
Subjects:	Animals Biological and medical sciences Carbon Tetrachloride - pharmacology Chemical and industrial products toxicology. Toxic occupational diseases Cytochrome P-450 CYP1A2 Cytochrome P-450 Enzyme System - metabolism Cytochromes - metabolism In Vitro Techniques Lipid Peroxides - metabolism Liver - drug effects Liver - enzymology Male Medical sciences Methylcholanthrene - pharmacology Mixed Function Oxygenases - metabolism Pentobarbital - pharmacology Rats Rats, Inbred Strains Solvents Toxicology
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container_title	Japanese Journal of Pharmacology
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creator	MASUDA, Yasusuke YASOSHIMA, Mitsue
description	In the cytochrome P-450-reconstituted system, CCI4 stimulated NADPH-dependent lipid peroxidation of the system containing the P-450 form to a much greater extent than that of the system containing the P-448 form. When the P-450-reconstituted system was preincubated in the presence of both NADPH and CCI4, 7-ethoxycoumarin O-deethylase, aminopyrine N-demethylase and aniline hydroxylase activities were decreased by 40–60%, whereas, with P-448 form reconstituted system, no suppression was observed in these enzyme activities or in 7-ethoxyresorufin O-deethylase activity. These observations suggest that the P-450 form, but not the P-448 form, is active in metabolizing CCI4 to a reactive species that subsequently impairs the hemoprotein.
doi_str_mv	10.1016/S0021-5198(19)43543-9
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Toxic occupational diseases</subject><subject>Cytochrome P-450 CYP1A2</subject><subject>Cytochrome P-450 Enzyme System - metabolism</subject><subject>Cytochromes - metabolism</subject><subject>In Vitro Techniques</subject><subject>Lipid Peroxides - metabolism</subject><subject>Liver - drug effects</subject><subject>Liver - enzymology</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Methylcholanthrene - pharmacology</subject><subject>Mixed Function Oxygenases - metabolism</subject><subject>Pentobarbital - pharmacology</subject><subject>Rats</subject><subject>Rats, Inbred Strains</subject><subject>Solvents</subject><subject>Toxicology</subject><issn>0021-5198</issn><issn>1347-3506</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1987</creationdate><recordtype>article</recordtype><recordid>eNqFkE1vEzEQhlcIVELhJ1TyAaFyWPDn2j6hKqKAVERE27Pl9c42jjZ2sL2ISPx4nCbqlYs91jzzjvw0zQXBHwgm3cdbjClpBdHqkuj3nAnOWv2sWRDGZcsE7p43iyfkZfMq5019Kkz4WXPGhGScykXz98oVHwOKI1ra1NfqDkqybj3F5AdAPqCyBvQTXAy5-DIXGND3GGL8s3-AYDOg230usEX32YcHtLKpeDtNe7Sakx99pZf7Et06xS2gVcsFRjYMh4qr182L0U4Z3pzu8-b--vPd8mt78-PLt-XVTesYU7pllGqCu95S2tG-k5KqjmLHuMJjj6kVWFE1jsL21DIMeNBSjp1SgspOOS3YefPumLtL8dcMuZitzw6myQaIczZScsW5VhUUR9ClmHOC0eyS39q0NwSbg3XzaN0clBqizaN1o-vcxWnB3G9heJo6aa79t6e-zc5OY7LB-fyEKcYF07Ri10esZvjKxTD5AGYT5xSqH-PGbrOJu6muVtJgzBmmBhNhcDVzOHRVQjtWgz4dg6Ba_e0hmew8BFdjE7hihuj_86N_mbaypA</recordid><startdate>1987</startdate><enddate>1987</enddate><creator>MASUDA, Yasusuke</creator><creator>YASOSHIMA, Mitsue</creator><general>The Japanese Pharmacological Society</general><general>Japanese Pharmacological Society</general><scope>6I.</scope><scope>AAFTH</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>1987</creationdate><title>Action of Carbon Tetrachloride in the Reconstituted Monooxygenase System Using Partially Purified Cytochrome P-450 and P-448</title><author>MASUDA, Yasusuke ; YASOSHIMA, Mitsue</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3389-3229106ba2262b67728620c3480fb02a50828ff5ab2a30e0d977f68852768c953</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1987</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Carbon Tetrachloride - pharmacology</topic><topic>Chemical and industrial products toxicology. 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subjects	Animals Biological and medical sciences Carbon Tetrachloride - pharmacology Chemical and industrial products toxicology. Toxic occupational diseases Cytochrome P-450 CYP1A2 Cytochrome P-450 Enzyme System - metabolism Cytochromes - metabolism In Vitro Techniques Lipid Peroxides - metabolism Liver - drug effects Liver - enzymology Male Medical sciences Methylcholanthrene - pharmacology Mixed Function Oxygenases - metabolism Pentobarbital - pharmacology Rats Rats, Inbred Strains Solvents Toxicology
title	Action of Carbon Tetrachloride in the Reconstituted Monooxygenase System Using Partially Purified Cytochrome P-450 and P-448
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