Identification of anticoagulant activities in the salivary glands of the soft tick, Ornithodoros savignyi

Salivary gland extracts of the sand tampan, Ornithodoros savignyi, prolonged the activated partial thromboplastin time (APTT) and prothrombin time (PT) significantly in a concentration-dependent manner. There was also a pronounced inhibition of human activated factor Xa (fXa) by salivary gland extra...

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Bibliographic Details
Published in:Experimental & applied acarology 1995-02, Vol.19 (2), p.117-127
Main Authors: GASPAR, A. R. M. D, CRAUSE, J. C, NEITZ, A. W. H
Format: Article
Language:English
Subjects:
Acari
Animals
Anticoagulants - metabolism
Arachnida
Argasidae
Biochemistry. Physiology. Immunology
Biological and medical sciences
Factor Xa - metabolism
Factor Xa Inhibitors
Fundamental and applied biological sciences. Psychology
Humans
Invertebrates
Ornithodoros savignyi
Physiology. Development
Salivary Glands - metabolism
Ticks - metabolism
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Summary:Salivary gland extracts of the sand tampan, Ornithodoros savignyi, prolonged the activated partial thromboplastin time (APTT) and prothrombin time (PT) significantly in a concentration-dependent manner. There was also a pronounced inhibition of human activated factor Xa (fXa) by salivary gland extracts. The salivary gland extracts inhibited chromogenic assays specific for both fXa and thrombin. Sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) of the salivary gland proteins followed by elution of specific areas or bands from a polyvinylidene difluoride (PVDF)-membrane, showed that various anticoagulant factors are present when screened by means of the APTT assay. The most active component was associated with a band of M(r) of 14 kDa. Partial purification of this component was achieved using isoelectric focusing (IEF) and size-exclusion high-performance liquid chromatography (HPLC).
ISSN:0168-8162
1572-9702
DOI:10.1007/bf00052551