Allele-specific DNA identity patterns

A method of genetic analysis is presented which involves digestion of DNA with a single restriction enzyme (PvuII) and hybridisation with a mixture of five probes. Four of the five probes chosen recognise hypervariable regions (HVRs) of the human genome and hence an allele-specific DNA identity patt...

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Bibliographic Details
Published in:Human genetics 1987-04, Vol.75 (4), p.384-387
Main Authors: WAINSCOAT, J. S, PILKINGTON, S, PETO, T. E. A, BELL, J. I, HIGGS, D. R
Format: Article
Language:English
Subjects:
Biological and medical sciences
DNA Restriction Enzymes
Fundamental and applied biological sciences. Psychology
Gene Frequency
Genes. Genome
Globins - genetics
Humans
Insulin - genetics
Molecular and cellular biology
Molecular genetics
Oncogenes
Pedigree
Polymorphism, Restriction Fragment Length
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Summary:A method of genetic analysis is presented which involves digestion of DNA with a single restriction enzyme (PvuII) and hybridisation with a mixture of five probes. Four of the five probes chosen recognise hypervariable regions (HVRs) of the human genome and hence an allele-specific DNA identity pattern results. An advantage of this approach to genetic characterisation is that the complex identity patterns may be broken down into simple allelic systems of known chromosomal localization by hybridisation with the individual probes. Also different probes may be included in a combined probe designed for particular types of investigation.
ISSN:0340-6717
1432-1203
DOI:10.1007/BF00284114