Determination of dopexamine hydrochloride in human blood by high-performance liquid chromatography with electrochemical detection

• Published in: Journal of chromatography. B, Biomedical applications Biomedical applications, 1995-05, Vol.667 (2), p.283-290
• Main Authors: Baker, P.R., Gardner, J.J., Lockley, W.J.S., Wilkinson, D.
• Format: Article
• Language: English
• Subjects: Adrenergic beta-Agonists; Chromatography, High Pressure Liquid - methods; Chromatography, High Pressure Liquid - statistics & numerical data; Dopamine - analogs & derivatives; Dopamine - blood; Dopamine - pharmacokinetics; Dopamine Agonists; Drug Stability; Electrochemistry; Humans; Kinetics; Quality Control; Sensitivity and Specificity; Sulfites
• ISSN: 0378-4347; 1572-6495
• DOI: 10.1016/0378-4347(95)00031-D

A method is described for the determination of dopexamine hydrochloride at concentrations of 5 to 1000 ng/ml in human blood using electrochemical detection. The method uses a Hypersil ODS column and a mobile phase containing heptane sulphonate, orthophosphoric acid, diisopropylamine and disodium EDTA. Blood samples are stabilised immediately after collection by the use of dipotassium EDTA and a high concentration of sodium metabisulphite. The sample preparation procedure consists of a simple de-proteinisation with perchloric acid. The method is accurate, with inter-assay accuracies ranging from 100 to 104%, and is free of interference by blood from different individuals. Known and potential metabolites of dopexamine hydrochloride and a wide range of drugs do not interfere with the method. The method is precise with inter-assay coefficients on variation of 10.6% at 5 ng/ml and of less than 4.2% at higher concentrations. Stabilised blood samples may be stored for over six months at −25°C prior to analysis.