Outer Membrane Protein F (Porin) Preparation of Pseudomonas aeruginosa as a Protective Vaccine Against Heterologous Immunotype Strains in a Burned Mouse Model

Outer membrane (OM) protein F (porin) was purified by extraction from polyacrylamide gels of cell envelope proteins of the Pseudomonas aeruginosa PAO1 strain. Mice were immunized intramuscularly with 10 μg of protein F preparation on days 1 and 14 and then subjected to burn and challenge on day 28....

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Bibliographic Details
Published in:The Journal of infectious diseases 1987-06, Vol.155 (6), p.1282-1291
Main Authors: Matthews-Greer, Janice M., Gilleland, H. E.
Format: Article
Language:English
Subjects:
Animals
Antibodies
Antibodies, Bacterial - biosynthesis
Antiserum
Bacterial Outer Membrane Proteins - immunology
Bacterial Vaccines - immunology
Bacteriology
Biological and medical sciences
Burns - complications
Fundamental and applied biological sciences. Psychology
Immune response
Immunization
Immunoglobulin G - biosynthesis
Immunoglobulin M - biosynthesis
Infections
Lipopolysaccharides - immunology
Mice
Microbiology
Original Articles
Porins
Proteins
Pseudomonas aeruginosa
Pseudomonas aeruginosa - immunology
Pseudomonas Infections - complications
Pseudomonas Infections - prevention & control
Vaccination
Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies
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Summary:Outer membrane (OM) protein F (porin) was purified by extraction from polyacrylamide gels of cell envelope proteins of the Pseudomonas aeruginosa PAO1 strain. Mice were immunized intramuscularly with 10 μg of protein F preparation on days 1 and 14 and then subjected to burn and challenge on day 28. Protein F immunization afforded significant protection above that provided by PAO1 lipopolysaccharide (LPS) immunization against subsequent challenge with six of six heterologous LPS immunotype strains of P. aeruginosa. By an ELISA, the murine immune response revealed an IgG titer of 5,120 to protein F by day 30. Immunoblot analysis of antisera from protein F-immunized mice revealed bands with both protein F and protein H of cell envelopes of all immunotypes tested. Active immunization with OM protein H did not, however, afford significant protection to mice in this burned mouse model. These data show the efficacy of OM protein F as a protective vaccine in a murine model representative of human infection.
ISSN:0022-1899
1537-6613
DOI:10.1093/infdis/155.6.1282