Establishment and Characterization of a Malignant Epithelioid Hemangioendothelioma from Mouse Thyroid Tumor

A mouse epithelioid hemangioendothelioma (mEHE) cell line, passagable in vivo without TSH stimulation, was established from a thyroid tumor. Before establishment of the cell line, the primary thyroid tumor which was co-transplanted with a TSH-producing pituitary adenoma showed signs of hyperplasia a...

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Bibliographic Details
Published in:ENDOCRINE JOURNAL 1995, Vol.42(3), pp.341-350
Main Authors: HOSODA, CHIHIRO, OHTSURU, AKIRA, NAMBA, HIROYUKI, MOTOMURA, KATSUAKI, HARA, TAKESHI, NAKASHIMA, MASAHIRO, ITO, MASAHIRO, YOKORO, KENJIRO, YAMASHITA, SHUNICHI
Format: Article
Language:English
Subjects:
Animals
Blotting, Northern
Cell Differentiation
Cell Division
Cholecalciferol - pharmacology
Cyclic AMP - pharmacology
DNA, Neoplasm - biosynthesis
Endothelial cell
Endothelium, Vascular - pathology
Epithelioid hemangioendothelioma
Epithelium - pathology
Female
Gene Expression - drug effects
Genes, myc
Hemangioendothelioma, Epithelioid - pathology
Immunohistochemistry
Kinetics
Mice
Neoplasm Transplantation
Thyroid Neoplasms - pathology
Thyroid tumorigenesis
Thyrotropin - pharmacology
TSH
Tumor Cells, Cultured
Vitamin D
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Summary:A mouse epithelioid hemangioendothelioma (mEHE) cell line, passagable in vivo without TSH stimulation, was established from a thyroid tumor. Before establishment of the cell line, the primary thyroid tumor which was co-transplanted with a TSH-producing pituitary adenoma showed signs of hyperplasia and then transformed during several in vivo transplantable passages. The cell line which was established from an in vitro culture, was deficient in thyroid-specific differentiation function and had characteristics of endothelial cell origin such as vascular cavity formation and factor VIII expression. The cell growth of mEHE/CH1-5 cell lines was independent of TSH but was inhibited by c-AMP and vitamin D3. c-Myc proto-oncogene expression was also suppressed by vitamin D3 treatment. Both serum depletion and heparin treatment induced morphological changes in mEHE/CH 5 from an epithelial cell-like to an endothelial cell-like shape. Immunohistochemical analysis showed that epithelial membrane antigen expression was decreased and factor VIII expression was increased in relation to the morphological changes. In a further in vivo transplantation experiment, the histology of the mEHE/CH5 cell tumor had an angiosarcoma-like structure. These results indicate that this cell line established from a thyroid tumor possesses both epithelial and endothelial characteristics. The mEHE/CH5 cells might provide a good model for analyzing thyroid tumorigenesis and allow functional characterization of endothelial cells.
ISSN:0918-8959
1348-4540
DOI:10.1507/endocrj.42.341