Efficient Packaging of Readthrough RNA in ALV: Implications for Oncogene Transduction

Readthrough viral transcripts are present at relatively high levels in cells infected with avian leukosis virus. It has been proposed that they can function as intermediates in the transduction of proto-oncogenes by retroviruses. It is shown here, by the analysis of viruses containing a mutation in...

Full description

Saved in:
Bibliographic Details
Published in:Science (American Association for the Advancement of Science) 1987-05, Vol.236 (4803), p.845-848
Main Authors: Herman, Steven A., Coffin, John M.
Format: Article
Language:English
Subjects:
Animals
Avian leukosis
avian leukosis virus
Avian Sarcoma Viruses - genetics
Base Sequence
Biological and medical sciences
Biotechnology
DNA Replication
Fibroblasts
Fundamental and applied biological sciences. Psychology
Genes, Viral
Genetic mutation
Genetics
Genomes
Medical research
Microbiology
Nucleotides
Oncogenes
Proto oncogenes
Retrovirus
Retroviruses
RNA
RNA probes
RNA, Viral - genetics
Transduction
Transduction (Genetics)
Transduction, Genetic
Turkeys
Viral RNA
Virion - genetics
Virions
Virology
Virus Replication
Viruses
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Readthrough viral transcripts are present at relatively high levels in cells infected with avian leukosis virus. It has been proposed that they can function as intermediates in the transduction of proto-oncogenes by retroviruses. It is shown here, by the analysis of viruses containing a mutation in the AAUAAA polyadenylation signal, that read-through RNAs have the requisite properties to function as transduction intermediates: (i) readthrough RNAs were polyadenylated and packaged as efficiently as normal viral RNA, (ii) RNAs nearly 11.2 kilobases (3.5 kilobases larger than wild-type avian leukosis virus genomes) were present in virions of the mutant virus, and (iii) virus particles containing both readthrough and normal genomes were most likely infectious.
ISSN:0036-8075
1095-9203
DOI:10.1126/science.3033828