Inhibition of High Affinity Basic Fibroblast Growth Factor Binding by Oligonucleotides (∗)

Oligonucleotides can be used to inhibit the binding of basic fibroblast growth factor to cells. Though standard phosphodiester oligonucleotides show a slight inhibition of binding, the oligonucleotides with phosphorothioate internucleoside linkages have inhibition levels equivalent to that of the po...

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Bibliographic Details
Published in:The Journal of biological chemistry 1995-09, Vol.270 (37), p.21718-21721
Main Authors: Fennewald, Susan M., Rando, Robert F.
Format: Article
Language:English
Subjects:
Animals
Base Sequence
Binding, Competitive
Chlorocebus aethiops
Fibroblast Growth Factor 2 - metabolism
Heparin - pharmacology
Kinetics
Molecular Sequence Data
Oligodeoxyribonucleotides - chemistry
Oligodeoxyribonucleotides - pharmacology
Receptors, Fibroblast Growth Factor - drug effects
Receptors, Fibroblast Growth Factor - metabolism
Structure-Activity Relationship
Thionucleotides
Vero Cells
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Description
Summary:Oligonucleotides can be used to inhibit the binding of basic fibroblast growth factor to cells. Though standard phosphodiester oligonucleotides show a slight inhibition of binding, the oligonucleotides with phosphorothioate internucleoside linkages have inhibition levels equivalent to that of the polyanion heparin. Variations in sequence of the oligonucleotides does lead to differences in the inhibitory action of the oligonucleotides. This inhibition of basic fibroblast growth factor by phosphorothioate oligonucleotides may account for much of the published data on inhibition of various genes by proposed antisense oligonucleotides and needs to be taken into account when considering the mechanism of action of oligonucleotides in biological systems.
ISSN:0021-9258
1083-351X
DOI:10.1074/jbc.270.37.21718