A Novel T-Cell Differentiation Antigen Expressed in Immature Human Thymocytes and Neuroglial Cells

We report a novel human thymocyte differentiation antigen ICT-1 with a molecular weight of 49 kDa that is noncovalently associated with another 12-kDa protein. The ICT-1 antigen is expressed in 50-70% of total thymocytes, but not in resting or PHA-activated peripheral blood T-cells and bone marrow c...

Full description

Saved in:
Bibliographic Details
Published in:Cellular immunology 1995-10, Vol.165 (1), p.118-124
Main Authors: Bae, Young Mee, Kim, Tae Jin, Park, Weon Seo, Chung, Doo Hyun, Choi, Eun Young, Lee, Geon Kook, Song, Hyung Geun, Kim, Chul Woo, Kim, Byung Kook, Ahn, Hyo Seop, Park, Myoung Hee, Shin, Hee Young, Chi, Je Geun, Park, Seong Hoe
Format: Article
Language:English
Subjects:
Animals
Antibodies, Monoclonal - immunology
Antigens, CD - biosynthesis
Antigens, CD - immunology
Antigens, CD1
Antigens, Differentiation, T-Lymphocyte - chemistry
Antigens, Differentiation, T-Lymphocyte - physiology
Embryonic and Fetal Development - immunology
Humans
Immunohistochemistry
Membrane Glycoproteins - biosynthesis
Mice
Mice, Inbred BALB C
Molecular Weight
Neuroglia - cytology
Neuroglia - metabolism
Phytohemagglutinins - pharmacology
Thymus Gland - cytology
Thymus Gland - metabolism
Transfection - immunology
Tumor Cells, Cultured
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:We report a novel human thymocyte differentiation antigen ICT-1 with a molecular weight of 49 kDa that is noncovalently associated with another 12-kDa protein. The ICT-1 antigen is expressed in 50-70% of total thymocytes, but not in resting or PHA-activated peripheral blood T-cells and bone marrow cells. The thymocytes expressing ICT-1 antigen appear after the 18th week of gestation during fetal development. Since the distribution pattern of the ICT-1 antigen within thymus partly overlaps with that of the CD1 antigens, we investigated whether ICT-1 was one of the CD1 antigen family. However, the failure of anti-ICT-1 antibody to react with mouse L cells transfected with cDNA of CD1a, -b, and -c and the different histologic distribution patterns from that of CD1d strongly suggest that the anti-ICT-1 antibody recognizes an antigen distinct from CD1. Furthermore, ICT-1 is also expressed in human neuroglial cells such as oligodendroglioma, glioblastoma multiforme, Ewing's sarcoma, and cerebellar astrocyte. Hence we believe that the ICT-1 antigen may be a novel thymus-leukemia (TL) antigen or a nonclassical MHC class I antigen.
ISSN:0008-8749
1090-2163
DOI:10.1006/cimm.1995.1194