Cloning and Characterization of a Dispersed, Multicopy, X Chromosome Sequence in Drosophila melanogaster

We have isolated and characterized a dispersed middle repetitive DNA sequence from Drosophila melanogaster that is concentrated on the euchromatic portion of the X chromosome. In situ hybridization of the repeat unit to salivary gland chromosomes shows the sequence is distributed among approximately...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1987-05, Vol.84 (9), p.2843-2847
Main Authors: Waring, Gail L., Pollack, Jane C.
Format: Article
Language:English
Subjects:
Animals
Bacteriophages
Base Sequence
Biological and medical sciences
Biotechnology
Cloning, Molecular
Cytogenetics
DNA
Drosophila
Drosophila melanogaster
Drosophila melanogaster - genetics
Drosophilidae
Fundamental and applied biological sciences. Psychology
Genetic engineering
Genetic technics
Genomics
In situ hybridization
Methods. Procedures. Technologies
Molecular cloning
Nucleic Acid Hybridization
Salivary glands
Sequence homology
Sequence Homology, Nucleic Acid
Sex chromosomes
X Chromosome
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Summary:We have isolated and characterized a dispersed middle repetitive DNA sequence from Drosophila melanogaster that is concentrated on the euchromatic portion of the X chromosome. In situ hybridization of the repeat unit to salivary gland chromosomes shows the sequence is distributed among approximately 10 major and 20 minor X chromosomal sites. Based on DNA sequence analysis of homologous sequences from three different cytogenetic regions, the 372-base-pair repeat unit appears to be (A+T)-rich and noncoding and shows strong sequence conservation among units from different chromosomal regions. The nature and distribution of this sequence are suggestive of the hypothetical X chromosome DNA sequences thought to be involved in the primary establishment of sex determination and dosage compensation in Drosophila.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.84.9.2843