Loading…
Cloning and Characterization of a Dispersed, Multicopy, X Chromosome Sequence in Drosophila melanogaster
We have isolated and characterized a dispersed middle repetitive DNA sequence from Drosophila melanogaster that is concentrated on the euchromatic portion of the X chromosome. In situ hybridization of the repeat unit to salivary gland chromosomes shows the sequence is distributed among approximately...
Saved in:
| Published in: | Proceedings of the National Academy of Sciences - PNAS 1987-05, Vol.84 (9), p.2843-2847 |
|---|---|
| Main Authors: | , |
| Format: | Article |
| Language: | English |
| Subjects: | |
| Citations: | Items that cite this one |
| Online Access: | Get full text |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| cited_by | cdi_FETCH-LOGICAL-c516t-49730aef9eebf4dc074edb51a266e39edaacdf28f56b2f24ddbffc2a12262723 |
|---|---|
| cites | |
| container_end_page | 2847 |
| container_issue | 9 |
| container_start_page | 2843 |
| container_title | Proceedings of the National Academy of Sciences - PNAS |
| container_volume | 84 |
| creator | Waring, Gail L. Pollack, Jane C. |
| description | We have isolated and characterized a dispersed middle repetitive DNA sequence from Drosophila melanogaster that is concentrated on the euchromatic portion of the X chromosome. In situ hybridization of the repeat unit to salivary gland chromosomes shows the sequence is distributed among approximately 10 major and 20 minor X chromosomal sites. Based on DNA sequence analysis of homologous sequences from three different cytogenetic regions, the 372-base-pair repeat unit appears to be (A+T)-rich and noncoding and shows strong sequence conservation among units from different chromosomal regions. The nature and distribution of this sequence are suggestive of the hypothetical X chromosome DNA sequences thought to be involved in the primary establishment of sex determination and dosage compensation in Drosophila. |
| doi_str_mv | 10.1073/pnas.84.9.2843 |
| format | article |
| fullrecord | <record><control><sourceid>jstor_proqu</sourceid><recordid>TN_cdi_proquest_miscellaneous_77512694</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><jstor_id>29281</jstor_id><sourcerecordid>29281</sourcerecordid><originalsourceid>FETCH-LOGICAL-c516t-49730aef9eebf4dc074edb51a266e39edaacdf28f56b2f24ddbffc2a12262723</originalsourceid><addsrcrecordid>eNqFkUtv1DAUhSMEKkNhywIJyQvUVSfYjvPwogs05SUVsaALdtaNcz3jKrGDnSDaX49HM0SDhMTKks93H-eeLHvJaM5oXbwdHcS8EbnMeSOKR9mKUcnWlZD0cbailNfrRnDxNHsW4x2lVJYNPcvOCkYrWTerbLfpvbNuS8B1ZLODAHrCYB9gst4RbwiQaxtHDBG7S_Jl7ier_Xh_Sb4nOvjBRz8g-YY_ZnQaiXXkOqS_cWd7IAP24PwWYmr5PHtioI_44vieZ7cf3t9uPq1vvn78vHl3s9Ylq6a1kHVBAY1EbI3oNK0Fdm3JgFcVFhI7AN0Z3piyarnhoutaYzQHxnnFa16cZ1eHtuPcDthpdFOAXo3BDhDulQer_lac3amt_6kKKuqySvUXx_rgk6c4qcFGjX0ygn6Oqq5Lxisp_gsyURcsXTmB-QHU6TAxoFmWYVTtI1T7CFUjlFT7CFPB61MLC37MLOlvjjpEDb0J4LSNC9Zw0fCiPFlw335R_4xRZu77CX9NJ_P-CSb91UG_i5MPC8Alb1jxG60kyE0</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>14731106</pqid></control><display><type>article</type><title>Cloning and Characterization of a Dispersed, Multicopy, X Chromosome Sequence in Drosophila melanogaster</title><source>PubMed Central (Open Access)</source><source>JSTOR Archival Journals</source><creator>Waring, Gail L. ; Pollack, Jane C.</creator><creatorcontrib>Waring, Gail L. ; Pollack, Jane C.</creatorcontrib><description>We have isolated and characterized a dispersed middle repetitive DNA sequence from Drosophila melanogaster that is concentrated on the euchromatic portion of the X chromosome. In situ hybridization of the repeat unit to salivary gland chromosomes shows the sequence is distributed among approximately 10 major and 20 minor X chromosomal sites. Based on DNA sequence analysis of homologous sequences from three different cytogenetic regions, the 372-base-pair repeat unit appears to be (A+T)-rich and noncoding and shows strong sequence conservation among units from different chromosomal regions. The nature and distribution of this sequence are suggestive of the hypothetical X chromosome DNA sequences thought to be involved in the primary establishment of sex determination and dosage compensation in Drosophila.</description><identifier>ISSN: 0027-8424</identifier><identifier>EISSN: 1091-6490</identifier><identifier>DOI: 10.1073/pnas.84.9.2843</identifier><identifier>PMID: 3106978</identifier><identifier>CODEN: PNASA6</identifier><language>eng</language><publisher>Washington, DC: National Academy of Sciences of the United States of America</publisher><subject>Animals ; Bacteriophages ; Base Sequence ; Biological and medical sciences ; Biotechnology ; Cloning, Molecular ; Cytogenetics ; DNA ; Drosophila ; Drosophila melanogaster ; Drosophila melanogaster - genetics ; Drosophilidae ; Fundamental and applied biological sciences. Psychology ; Genetic engineering ; Genetic technics ; Genomics ; In situ hybridization ; Methods. Procedures. Technologies ; Molecular cloning ; Nucleic Acid Hybridization ; Salivary glands ; Sequence homology ; Sequence Homology, Nucleic Acid ; Sex chromosomes ; X Chromosome</subject><ispartof>Proceedings of the National Academy of Sciences - PNAS, 1987-05, Vol.84 (9), p.2843-2847</ispartof><rights>1987 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c516t-49730aef9eebf4dc074edb51a266e39edaacdf28f56b2f24ddbffc2a12262723</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://www.pnas.org/content/84/9.cover.gif</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/29281$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/29281$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,27924,27925,53791,53793,58238,58471</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=8248235$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/3106978$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Waring, Gail L.</creatorcontrib><creatorcontrib>Pollack, Jane C.</creatorcontrib><title>Cloning and Characterization of a Dispersed, Multicopy, X Chromosome Sequence in Drosophila melanogaster</title><title>Proceedings of the National Academy of Sciences - PNAS</title><addtitle>Proc Natl Acad Sci U S A</addtitle><description>We have isolated and characterized a dispersed middle repetitive DNA sequence from Drosophila melanogaster that is concentrated on the euchromatic portion of the X chromosome. In situ hybridization of the repeat unit to salivary gland chromosomes shows the sequence is distributed among approximately 10 major and 20 minor X chromosomal sites. Based on DNA sequence analysis of homologous sequences from three different cytogenetic regions, the 372-base-pair repeat unit appears to be (A+T)-rich and noncoding and shows strong sequence conservation among units from different chromosomal regions. The nature and distribution of this sequence are suggestive of the hypothetical X chromosome DNA sequences thought to be involved in the primary establishment of sex determination and dosage compensation in Drosophila.</description><subject>Animals</subject><subject>Bacteriophages</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>Cloning, Molecular</subject><subject>Cytogenetics</subject><subject>DNA</subject><subject>Drosophila</subject><subject>Drosophila melanogaster</subject><subject>Drosophila melanogaster - genetics</subject><subject>Drosophilidae</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genetic engineering</subject><subject>Genetic technics</subject><subject>Genomics</subject><subject>In situ hybridization</subject><subject>Methods. Procedures. Technologies</subject><subject>Molecular cloning</subject><subject>Nucleic Acid Hybridization</subject><subject>Salivary glands</subject><subject>Sequence homology</subject><subject>Sequence Homology, Nucleic Acid</subject><subject>Sex chromosomes</subject><subject>X Chromosome</subject><issn>0027-8424</issn><issn>1091-6490</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1987</creationdate><recordtype>article</recordtype><recordid>eNqFkUtv1DAUhSMEKkNhywIJyQvUVSfYjvPwogs05SUVsaALdtaNcz3jKrGDnSDaX49HM0SDhMTKks93H-eeLHvJaM5oXbwdHcS8EbnMeSOKR9mKUcnWlZD0cbailNfrRnDxNHsW4x2lVJYNPcvOCkYrWTerbLfpvbNuS8B1ZLODAHrCYB9gst4RbwiQaxtHDBG7S_Jl7ier_Xh_Sb4nOvjBRz8g-YY_ZnQaiXXkOqS_cWd7IAP24PwWYmr5PHtioI_44vieZ7cf3t9uPq1vvn78vHl3s9Ylq6a1kHVBAY1EbI3oNK0Fdm3JgFcVFhI7AN0Z3piyarnhoutaYzQHxnnFa16cZ1eHtuPcDthpdFOAXo3BDhDulQer_lac3amt_6kKKuqySvUXx_rgk6c4qcFGjX0ygn6Oqq5Lxisp_gsyURcsXTmB-QHU6TAxoFmWYVTtI1T7CFUjlFT7CFPB61MLC37MLOlvjjpEDb0J4LSNC9Zw0fCiPFlw335R_4xRZu77CX9NJ_P-CSb91UG_i5MPC8Alb1jxG60kyE0</recordid><startdate>19870501</startdate><enddate>19870501</enddate><creator>Waring, Gail L.</creator><creator>Pollack, Jane C.</creator><general>National Academy of Sciences of the United States of America</general><general>National Acad Sciences</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SS</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19870501</creationdate><title>Cloning and Characterization of a Dispersed, Multicopy, X Chromosome Sequence in Drosophila melanogaster</title><author>Waring, Gail L. ; Pollack, Jane C.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c516t-49730aef9eebf4dc074edb51a266e39edaacdf28f56b2f24ddbffc2a12262723</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1987</creationdate><topic>Animals</topic><topic>Bacteriophages</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>Cloning, Molecular</topic><topic>Cytogenetics</topic><topic>DNA</topic><topic>Drosophila</topic><topic>Drosophila melanogaster</topic><topic>Drosophila melanogaster - genetics</topic><topic>Drosophilidae</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genetic engineering</topic><topic>Genetic technics</topic><topic>Genomics</topic><topic>In situ hybridization</topic><topic>Methods. Procedures. Technologies</topic><topic>Molecular cloning</topic><topic>Nucleic Acid Hybridization</topic><topic>Salivary glands</topic><topic>Sequence homology</topic><topic>Sequence Homology, Nucleic Acid</topic><topic>Sex chromosomes</topic><topic>X Chromosome</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Waring, Gail L.</creatorcontrib><creatorcontrib>Pollack, Jane C.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Waring, Gail L.</au><au>Pollack, Jane C.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cloning and Characterization of a Dispersed, Multicopy, X Chromosome Sequence in Drosophila melanogaster</atitle><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle><addtitle>Proc Natl Acad Sci U S A</addtitle><date>1987-05-01</date><risdate>1987</risdate><volume>84</volume><issue>9</issue><spage>2843</spage><epage>2847</epage><pages>2843-2847</pages><issn>0027-8424</issn><eissn>1091-6490</eissn><coden>PNASA6</coden><abstract>We have isolated and characterized a dispersed middle repetitive DNA sequence from Drosophila melanogaster that is concentrated on the euchromatic portion of the X chromosome. In situ hybridization of the repeat unit to salivary gland chromosomes shows the sequence is distributed among approximately 10 major and 20 minor X chromosomal sites. Based on DNA sequence analysis of homologous sequences from three different cytogenetic regions, the 372-base-pair repeat unit appears to be (A+T)-rich and noncoding and shows strong sequence conservation among units from different chromosomal regions. The nature and distribution of this sequence are suggestive of the hypothetical X chromosome DNA sequences thought to be involved in the primary establishment of sex determination and dosage compensation in Drosophila.</abstract><cop>Washington, DC</cop><pub>National Academy of Sciences of the United States of America</pub><pmid>3106978</pmid><doi>10.1073/pnas.84.9.2843</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0027-8424 |
| ispartof | Proceedings of the National Academy of Sciences - PNAS, 1987-05, Vol.84 (9), p.2843-2847 |
| issn | 0027-8424 1091-6490 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_77512694 |
| source | PubMed Central (Open Access); JSTOR Archival Journals |
| subjects | Animals Bacteriophages Base Sequence Biological and medical sciences Biotechnology Cloning, Molecular Cytogenetics DNA Drosophila Drosophila melanogaster Drosophila melanogaster - genetics Drosophilidae Fundamental and applied biological sciences. Psychology Genetic engineering Genetic technics Genomics In situ hybridization Methods. Procedures. Technologies Molecular cloning Nucleic Acid Hybridization Salivary glands Sequence homology Sequence Homology, Nucleic Acid Sex chromosomes X Chromosome |
| title | Cloning and Characterization of a Dispersed, Multicopy, X Chromosome Sequence in Drosophila melanogaster |
| url | http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-29T19%3A13%3A53IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-jstor_proqu&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Cloning%20and%20Characterization%20of%20a%20Dispersed,%20Multicopy,%20X%20Chromosome%20Sequence%20in%20Drosophila%20melanogaster&rft.jtitle=Proceedings%20of%20the%20National%20Academy%20of%20Sciences%20-%20PNAS&rft.au=Waring,%20Gail%20L.&rft.date=1987-05-01&rft.volume=84&rft.issue=9&rft.spage=2843&rft.epage=2847&rft.pages=2843-2847&rft.issn=0027-8424&rft.eissn=1091-6490&rft.coden=PNASA6&rft_id=info:doi/10.1073/pnas.84.9.2843&rft_dat=%3Cjstor_proqu%3E29281%3C/jstor_proqu%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c516t-49730aef9eebf4dc074edb51a266e39edaacdf28f56b2f24ddbffc2a12262723%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=14731106&rft_id=info:pmid/3106978&rft_jstor_id=29281&rfr_iscdi=true |