Enhanced oxidative burst without interleukin 1 production by normal human polymorphonuclear leukocytes primed with muramyl dipeptides

Purified polymorphonuclear (PMN) cells were obtained from human blood leukocytes by centrifugation on colloidal silica gradients. PMNs could be primed for PMA-triggered oxidative burst by muramyl peptide molecules (MDP) and two of its adjuvant active nonpyrogenic derivatives. The priming effect of M...

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Bibliographic Details
Published in:Inflammation 1987-06, Vol.11 (2), p.153-161
Main Authors: JUPIN, C, PARANT, M, CHEDID, L, DAMAIS, C
Format: Article
Language:English
Subjects:
Acetylmuramyl-Alanyl-Isoglutamine - analogs & derivatives
Acetylmuramyl-Alanyl-Isoglutamine - pharmacology
Biological and medical sciences
Humans
Immunomodulators
In Vitro Techniques
Interleukin-1 - biosynthesis
Medical sciences
Neutrophils - drug effects
Neutrophils - immunology
Neutrophils - metabolism
Pharmacology. Drug treatments
Superoxides - metabolism
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Summary:Purified polymorphonuclear (PMN) cells were obtained from human blood leukocytes by centrifugation on colloidal silica gradients. PMNs could be primed for PMA-triggered oxidative burst by muramyl peptide molecules (MDP) and two of its adjuvant active nonpyrogenic derivatives. The priming effect of MDP could be demonstrated after a 1-h incubation period, whereas monocytes needed an 18-h incubation to produce an enhanced response in the NBT reduction test. Only the monocyte-enriched population was able to produce IL-1 activity after muramyl peptide stimulation. Under such conditions, PMNs neither produced nor secreted IL-1-like activity, and no IL-1 inhibitor was present in the supernatant fluids. In conclusion, muramyl peptides were able to prime PMNs for oxidative burst but not to stimulate IL-1-like factor production.
ISSN:0360-3997
1573-2576
DOI:10.1007/bf00916017