Inhibition of Renin Release by Analogues of Adenosine in Rabbit Renal Cortical Slices

• Published in: Hypertension (Dallas, Tex. 1979) Tex. 1979), 1987-06, Vol.9 (6), p.619-623
• Main Authors: BARCHOWSKY, AARON, DATA, JOANN L, WHORTON, A RICHARD
• Format: Article
• Language: English
• Subjects: Adenosine - analogs & derivatives; Adenosine - pharmacology; Adenosine-5'-(N-ethylcarboxamide); Animals; Biological and medical sciences; Deoxyadenosines - pharmacology; Isomerism; Isoproterenol - pharmacology; Kidney Cortex - metabolism; Male; Medical sciences; Ophthalmology; Phenylisopropyladenosine - pharmacology; Rabbits; Receptors, Adrenergic, beta - drug effects; Renin - metabolism; Theophylline - analogs & derivatives; Theophylline - pharmacology
• ISSN: 0194-911X; 1524-4563
• DOI: 10.1161/01.HYP.9.6.619

Renal cortical slices obtained from male New Zealand rabbits were used to investigate the role of adenosine in the regulation of renin release. Isoproterenol produced a significant (p < 0.01), twofold to threefold increase in renin release, that was both dose-dependent and time-dependent. Addition of either the-phenylisopropyl or the N-ethylcarboxamido derivative of adenosine attenuated this stimulation at concentrations as low as 10 M or 10 M, respectively. Higher doses of d-phenylisopropyladenosine (10 M) or adenosine (10 M) were necessary to significantly reduce the β-adrenergic response (p < 0.01). Inhibition was absent in slices preincubated with 10 M 8-phenyltheophylline, a concentration that had no effect on either basal or stimulated renin release. The site of inhibition appeared to be distal to β-adrenergic and prostaglandin receptors since-phenylisopropyladenosine (10 M) blocked stimulation by selective β-adrenergic receptor agonists, prenalterol (10 M) or salbutamol (10 M), and by prostaglandin E,. These data suggest that adenosine and its analogues inhibit renin release and that this inhibition may be mediated by a receptor-dependent action on a common point in the pathway leading to release.