Crystal structure of a TFIIB-TBP-TATA-element ternary complex

The crystal structure of the transcription factorIIB (TFIIB)/TATA box-binding protein (TBP)/ TATA-element ternary complex is described at 2.7 Å resolution. Core TFIIB resembles cyclin A, and recognizes the preformed TBP–DNA complex through protein–protein and protein–DNA interactions. The amino-term...

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Bibliographic Details
Published in:Nature (London) 1995-09, Vol.377 (6545), p.119-128
Main Authors: Nikolov, Dimitar B, Chen, Hua, Halay, Elaine D, Usheva, Anny A, Hisatake, Koji, Lee, Dong Kun, Roeder, Robert G, Burley, Stephen K
Format: Article
Language:English
Subjects:
Adenoviridae - genetics
Amino Acid Sequence
Arabidopsis
Base Sequence
Biochemistry
Biological and medical sciences
Crystal structure
Crystallography
Crystallography, X-Ray
Deoxyribonucleic acid
DNA
DNA-Binding Proteins - chemistry
Escherichia coli
Fundamental and applied biological sciences. Psychology
Humanities and Social Sciences
Humans
Models, Molecular
Molecular and cellular biology
Molecular biology
Molecular genetics
Molecular Sequence Data
multidisciplinary
Nucleic Acid Conformation
Promoter Regions, Genetic
Protein Binding
Protein Conformation
Proteins
Recognition
Recombinant Proteins
Science
Science (multidisciplinary)
Surface chemistry
TATA Box
TATA-Box Binding Protein
Transcription Factor TFIIB
Transcription Factors - chemistry
Transcription. Transcription factor. Splicing. Rna processing
Transcriptional Activation
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Summary:The crystal structure of the transcription factorIIB (TFIIB)/TATA box-binding protein (TBP)/ TATA-element ternary complex is described at 2.7 Å resolution. Core TFIIB resembles cyclin A, and recognizes the preformed TBP–DNA complex through protein–protein and protein–DNA interactions. The amino-terminal domain of core TFIIB forms the downstream surface of the ternary complex, where it could fix the transcription start site. The remaining surfaces of TBP and the TFIIB can interact with TBP-associated factors, other class II initiation factors, and transcriptional activators and coactivators.
ISSN:0028-0836
1476-4687
DOI:10.1038/377119a0