Identification of a Putative Steroidogenic Factor-1 Response Element in the DAX-1 Promoter

• Published in: Biochemical and biophysical research communications 1995-09, Vol.214 (2), p.576-581
• Main Authors: Burris, T.P., Guo, W.W., Le, T., Mccabe, E.R.B.
• Format: Article
• Language: English
• Subjects: Base Sequence; Binding Sites; Cell Nucleus - metabolism; Consensus Sequence; DAX-1 Orphan Nuclear Receptor; DNA-Binding Proteins - biosynthesis; DNA-Binding Proteins - genetics; Fushi Tarazu Transcription Factors; Homeodomain Proteins; Humans; Molecular Sequence Data; Oligodeoxyribonucleotides; Polymerase Chain Reaction; Promoter Regions, Genetic; Receptors, Cytoplasmic and Nuclear - biosynthesis; Receptors, Cytoplasmic and Nuclear - genetics; Receptors, Cytoplasmic and Nuclear - metabolism; Receptors, Retinoic Acid - biosynthesis; Receptors, Retinoic Acid - genetics; Repressor Proteins; Sequence Homology, Nucleic Acid; Steroidogenic Factor 1; TATA Box; Transcription Factors - biosynthesis; Transcription Factors - genetics; Transcription Factors - metabolism
• ISSN: 0006-291X; 1090-2104
• DOI: 10.1006/bbrc.1995.2324

The nuclear hormone receptor, DAX-1, is responsible for X-linked adrenal hypoplasia congenita and hypogonadotrophic hypogonadism. We recently cloned the 5′ flanking region of the human DAX-1 gene and in this report we describe the identification of a putative steroidogenic factor 1 (SF-1) response element approximately 110 bases upstream of the TATA box. Both DAX-1 and SF-1 are expressed in similar tissues including the adrenal cortex, gonads. hypothalamus, and the pituitary gland. Like DAX-1, SF-1 expression has been shown to be essential for the development of the adrenal cortex. We demonstrate that SF-1 is able to efficiently bind to the putative SF-1 response element found in the DAX-1 promoter in vitro. This suggests that SF-1 may directly regulate the expression of DAX-1 and that these two transcription factors may be components of a cascade required for development of steroidogenic tissues.