Tethered Benzophenone Reagents for the Synthesis of Photoactivatable Ligands

A new radiolabeled, bifunctional photoaffinity cross-linking reagent, N-succinimidyl p-benzoyl-[2,3-3H2]dihydrocinnamate, has been synthesized in high yield and with high specific activity. This reagent can be used to append the benzophenone photophore to amino groups of small molecules, such as O-a...

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Bibliographic Details
Published in:Bioconjugate chemistry 1995-07, Vol.6 (4), p.395-400
Main Authors: Olszewski, John D, Dorman, Gyorgy, Elliott, John T, Hong, Yang, Ahern, David G, Prestwich, Glenn D
Format: Article
Language:English
Subjects:
Affinity Labels - chemical synthesis
Affinity Labels - chemistry
Benzophenones - chemical synthesis
Benzophenones - chemistry
Cross-Linking Reagents - chemical synthesis
Cross-Linking Reagents - chemistry
Indicators and Reagents
Inositol Phosphates
Isotope Labeling
Ligands
Magnetic Resonance Spectroscopy
Peptides
Photochemistry
Spectrophotometry, Ultraviolet
Succinimides - chemical synthesis
Succinimides - chemistry
Tritium
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Summary:A new radiolabeled, bifunctional photoaffinity cross-linking reagent, N-succinimidyl p-benzoyl-[2,3-3H2]dihydrocinnamate, has been synthesized in high yield and with high specific activity. This reagent can be used to append the benzophenone photophore to amino groups of small molecules, such as O-aminoalkylinositol polyphosphates and polypeptides. The resulting tritiated photoaffinity labels can be purified and manipulated in ambient light and can be activated at 360 nm.
ISSN:1043-1802
1520-4812
DOI:10.1021/bc00034a009