Use of Polar Aprotic Solvents to Release Membranes from Milk Lipid Globules

In the search for alternatives to physical methods for release of membranes from milk lipid globules, aqueous solutions of the polar aprotic solvents dimethyl sulfoxide and dimethyl formamide were found to cause release of milk fat globule membrane. Membranes released with polar, aprotic solvents di...

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Bibliographic Details
Published in:Journal of dairy science 1987-04, Vol.70 (4), p.760-765
Main Authors: Dapper, Christie H., Valivullah, H. Mohammed, Keenan, T.W.
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Cattle
dimethyl formamide
Dimethyl Sulfoxide - pharmacology
Dimethylformamide - pharmacology
DMSO
ENZIMAS
ENZYME
ENZYMES
FAT GLOBULES
Food industries
Fundamental and applied biological sciences. Psychology
GLOBULE GRAS
GLOMERULOS DE GRASA
In Vitro Techniques
LAIT
LECHE
LIPIDE
LIPIDOS
LIPIDS
Membrane Lipids - isolation & purification
MILK
Milk - drug effects
Milk and cheese industries. Ice creams
PEPTIDE
PEPTIDES
PEPTIDOS
SOLVANT
SOLVENTE
SOLVENTS
Solvents - pharmacology
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Summary:In the search for alternatives to physical methods for release of membranes from milk lipid globules, aqueous solutions of the polar aprotic solvents dimethyl sulfoxide and dimethyl formamide were found to cause release of milk fat globule membrane. Membranes released with polar, aprotic solvents displayed some differences in polypeptide, enzymatic, and lipid composition in comparison with membranes released by churning. However, all polypeptide, enzymatic, and lipid constituents measured in membranes released by churning were also present in membranes released with dimethyl formamide and dimethyl sulfoxide. Antigenic and Concanavalin A binding activity of polypeptides was retained during exposure to dimethyl sulfoxide or dimethyl formamide. The solvent method was effective in releasing membrane from lipid globules of cow, rat, and guinea pig milks. This solvent method was rapid and minimized losses attendant with physical methods for release of milk fat globule membrane.
ISSN:0022-0302
1525-3198
DOI:10.3168/jds.S0022-0302(87)80071-1